Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of mouse brain using 12716-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12716-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12716-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 12716-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 12716-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12716-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12716-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12716-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12716-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12716-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12716-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12716-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 12716-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12716-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12716-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 12716-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 12716-1-AP
IP result of anti-PPID (IP:12716-1-AP, 4ug; Detection:12716-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human cervical cancer using 12716-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12716-1-AP (PPID antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse testis using 12716-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12716-1-AP (PPID antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 12716-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12716-1-AP (PPID antibody) at dilution of 1:50 (under 40x lens).
IF Staining of K-562 using 12716-1-AP
Immunofluorescent analysis of (4% PFA) fixed K-562 cells using PPID antibody (12716-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

PPID Polyclonal antibody

PPID Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12716-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

CYP40, Cyclophilin-related protein, Cyclophilin-40, Cyclophilin D, Cyclophilin 40

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, A549 cells, human brain tissue, Jurkat cells, MCF-7 cells, NIH/3T3 cells, Raji cells, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse testis tissue, human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	K-562 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 12 publications below
IF	See 1 publications below
CoIP	See 3 publications below

Product Information

12716-1-AP targets PPID in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3409 Product name: Recombinant human PPID protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 57-370 aa of BC030707 Sequence: GIGHTTGKPLHFKGCPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGRNTNGSQFFITTVPTPHLDGKHVVFGQVIKGIGVARILENVEVKGEKPAKLCVIAECGELKEGDDGGIFPKDGSGDSHPDFPEDADIDLKDVDKILLITEDLKNIGNTFFKSQNWEMAIKKYAEVLRYVDSSKAVIETADRAKLQPIALSCVLNIGACKLKMSNWQGAIDSCLEALELDPSNTKALYRRAQGWQGLKEYDQALADLKKAQGIAPEDKAIQAELLKVKQKIKAQKDKEKAVYAKMFA 相同性解析による交差性が予測される生物種
Full Name	peptidylprolyl isomerase D
Calculated molecular weight	370 aa, 41 kDa
Observed molecular weight	38-41 kDa
GenBank accession number	BC030707
Gene Symbol	PPID
Gene ID (NCBI)	5481
RRID	AB_2237551
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q08752
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228).

Protocols

Product Specific Protocols
IF protocol for PPID antibody 12716-1-AP	Download protocol
IHC protocol for PPID antibody 12716-1-AP	Download protocol
IP protocol for PPID antibody 12716-1-AP	Download protocol
WB protocol for PPID antibody 12716-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Front Mol Neurosci PMID: 29632473 Authors - Jian Zhang View Article
rat	WB,CoIP	Cardiovasc Toxicol PM2.5-Induced Programmed Myocardial Cell Death via mPTP Opening Results in Deteriorated Cardiac Function in HFpEF Mice. Authors - Tingting Wu View Article
human	WB	Mol Med Rep Downregulation of microRNA‑199a‑5p attenuates hypoxia/reoxygenation‑induced cytotoxicity in cardiomyocytes by targeting the HIF‑1α‑GSK3β‑mPTP axis. Authors - Da-Wei Liu View Article
human	WB	Eur J Pharmacol A novel ent-kaurane diterpenoid analog, DN3, selectively kills human gastric cancer cells via acting directly on mitochondria. Authors - Yong-Cheng Ma View Article
human	WB	Molecules The Mechanism of a Novel Mitochondrial-Targeted Icaritin Derivative in Regulating Apoptosis of BEL-7402 Cells Based on the SIRT3 and CypD-Mediated ROS/p38 MAPK Signaling Pathway Authors - Zenan Chen View Article
human	WB,CoIP	J Periodontal Res Porphyromonas gingivalis Induces Endothelial Dysfunction Through Sirt3-Dependent CypD Acetylation Authors - Shengming Xu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse brain using 12716-1-AP

WB analysis of Jurkat using 12716-1-AP

WB analysis of human brain using 12716-1-AP

WB analysis of rat brain using 12716-1-AP

WB analysis of Raji using 12716-1-AP

WB analysis of human brain using 12716-1-AP

WB analysis of human brain using 12716-1-AP

WB analysis of human brain using 12716-1-AP

WB analysis of Jurkat using 12716-1-AP

WB analysis of A549 using 12716-1-AP

WB analysis of A549 using 12716-1-AP

WB analysis of A549 using 12716-1-AP

WB analysis of Raji using 12716-1-AP

WB analysis of MCF-7 using 12716-1-AP

WB analysis of MCF-7 using 12716-1-AP

WB analysis of NIH/3T3 using 12716-1-AP

IP experiment of mouse brain using 12716-1-AP

IHC staining of human cervical cancer using 12716-1-AP

IHC staining of mouse testis using 12716-1-AP

IHC staining of human cervical cancer using 12716-1-AP

IF Staining of K-562 using 12716-1-AP

PPID Polyclonal antibody

PPID Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

PMID: 29632473

PM2.5-Induced Programmed Myocardial Cell Death via mPTP Opening Results in Deteriorated Cardiac Function in HFpEF Mice.

Downregulation of microRNA‑199a‑5p attenuates hypoxia/reoxygenation‑induced cytotoxicity in cardiomyocytes by targeting the HIF‑1α‑GSK3β‑mPTP axis.

A novel ent-kaurane diterpenoid analog, DN3, selectively kills human gastric cancer cells via acting directly on mitochondria.

The Mechanism of a Novel Mitochondrial-Targeted Icaritin Derivative in Regulating Apoptosis of BEL-7402 Cells Based on the SIRT3 and CypD-Mediated ROS/p38 MAPK Signaling Pathway

Porphyromonas gingivalis Induces Endothelial Dysfunction Through Sirt3-Dependent CypD Acetylation