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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

POU3F3-Specific Polyclonal antibody

POU3F3-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18999-1-AP

Synonyms

Brain 1, Brn 1, BRN1, Oct 8, Octamer binding protein 8, OTF 8, OTF8, POU class 3 homeobox 3, POU3F3

Validation Data Gallery

Applications:
  • WB analysis of SH-SY5Y using 18999-1-AP

    WB analysis of SH-SY5Y using 18999-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 18999-1-AP

    WB analysis of SH-SY5Y using 18999-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 18999-1-AP

    WB analysis of SH-SY5Y using 18999-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 18999-1-AP

    WB analysis of mouse brain using 18999-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 18999-1-AP

    WB analysis of mouse brain using 18999-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 18999-1-AP

    WB analysis of human brain using 18999-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 18999-1-AP

    WB analysis of mouse brain using 18999-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 18999-1-AP

    WB analysis of HEK-293 using 18999-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 18999-1-AP

    WB analysis of HEK-293 using 18999-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 18999-1-AP

    WB analysis of SH-SY5Y using 18999-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 18999-1-AP

    WB analysis of HEK-293 using 18999-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 18999-1-AP

    WB analysis of HEK-293 using 18999-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 18999-1-AP

    IHC staining of human brain using 18999-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 18999-1-AP

    IHC staining of human brain using 18999-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inSH-SY5Y cells, HEK-293 cells, human brain tissue, mouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

18999-1-AP targets POU3F3-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name POU class 3 homeobox 3
Calculated molecular weight 50 kDa
Observed molecular weight 66 kDa
GenBank accession numberNM_006236
Gene symbol POU3F3
Gene ID (NCBI) 5455
RRIDAB_2284130
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OTF8, also named as POU3F3 and BRN1, belongs to the POU transcription factor family and Class-3 subfamily. The antibody is specific to OTF8.

Protocols

Product Specific Protocols
WB protocol for POU3F3-Specific antibody 18999-1-APDownload protocol
IHC protocol for POU3F3-Specific antibody 18999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Dev Cell

AP-2β/KCTD1 Control Distal Nephron Differentiation and Protect against Renal Fibrosis.

Authors - Alexander G Marneros
View Article
humanWB

Int Immunopharmacol

Iguratimod-encapsulating PLGA-NPs induce human multiple myeloma cell death via reactive oxygen species and Caspase-dependent signalling.

Authors - Faming Wang
View Article
humanIHC

Histochem Cell Biol

Connecting tubules develop from the tip of the ureteric bud in the human kidney.

Authors - Beatrix Sarkany
View Article