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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

POLH Polyclonal antibody

POLH Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 28133-1-AP

Synonyms

DNA polymerase eta, FLJ16395, FLJ21978, POLH, polymerase (DNA directed), eta, RAD30, RAD30 homolog A, RAD30A, XP V, XPV

Validation Data Gallery

Applications:
  • WB analysis of BxPC-3 using 28133-1-AP

    WB analysis of BxPC-3 using 28133-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 28133-1-AP

    WB analysis of HeLa using 28133-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 28133-1-AP

    WB analysis of COLO 320 using 28133-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293T using 28133-1-AP

    WB analysis of HEK-293T using 28133-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells, COLO 320 cells, HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

28133-1-AP targets POLH in WB,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLH fusion protein Ag27973 相同性解析による交差性が予測される生物種
Full Name polymerase (DNA directed), eta
Calculated molecular weight713aa,78 kDa; 414aa,46 kDa
Observed molecular weight 78-85 kDa
GenBank accession numberBC015742
Gene symbol POLH
Gene ID (NCBI) 5429
RRIDAB_2881070
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for POLH antibody 28133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Katherine (Verified Customer) (10-31-2023)

The quality was good. Band was around 70kD

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: 293T
POLH Antibody Western Blot validation (1:500 dilution) in 293T (Cat no:28133-1-AP)