Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 15080-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15080-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15080-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 15080-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15080-1-AP
IP result of anti-POLDIP2 (IP:15080-1-AP, 4ug; Detection:15080-1-AP 1:1000) with HeLa cells lysate 1600ug.
IHC staining of human stomach cancer using 15080-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15080-1-AP (POLDIP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 15080-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15080-1-AP (POLDIP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15080-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLDIP2 antibody (15080-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

POLDIP2 Polyclonal antibody

POLDIP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15080-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

HSPC017, p38, PDIP38, POLD4, Polymerase delta-interacting protein 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells, HepG2 cells, K-562 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IF	See 1 publications below

Product Information

15080-1-AP targets POLDIP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7154 Product name: Recombinant human POLDIP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-368 aa of BC000655 Sequence: MAACTARRALAVGSRWWSRSLTGARWPRPLCAAAGAGAFSPASTTTTRRHLSSRNRPEGKVLETVGVFEVPKQNGKYETGQLFLHSIFGYRGVVLFPWQARLYDRDVASAAPEKAENPAGHGSKEVKGKTHTYYQVLIDARDCPHISQRSQTEAVTFLANHDDSRALYAIPGLDYVSHEDILPYTSTDQVPIQHELFERFLLYDQTKAPPFVARETLRAWQEKNHPWLELSDVHRETTENIRVTVIPFYMGMREAQNSHVYWWRYCIRLENLDSDVVQLRERHWRIFSLSGTLETVRGRGVVGREPVLSKEQPAFQYSSHVSLQASSGHMWGTFRFERPDGSHFDVRIPPFSLESNKDEKTPPSGLHW 相同性解析による交差性が予測される生物種
Full Name	polymerase (DNA-directed), delta interacting protein 2
Calculated molecular weight	42 kDa
Observed molecular weight	38 kDa
GenBank accession number	BC000655
Gene Symbol	POLDIP2
Gene ID (NCBI)	26073
RRID	AB_2166599
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y2S7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

POLDIP2(Polymerase delta-interacting protein 2) is also named as PDIP38, POLD4. It is as a unique positive regulator of Nox4 via its association with p22phox and together with Nox4, has profound effects on Rhodependent cytoskeletal remodeling. It is also as a regulator of cell division. It consists of 368 amino acids and has a predicted molecular mass of 42 kDa, with a potential signal peptide cleavage site after the first N-terminal 48 residues, which would result in a protein of 37 kDa(PMID:19574552). Endogenous human and rat POLDIP2 localized to the nucleus, plasma membrane, and cytoplasm(PMID:17623671).

Protocols

Product Specific Protocols
IF protocol for POLDIP2 antibody 15080-1-AP	Download protocol
IHC protocol for POLDIP2 antibody 15080-1-AP	Download protocol
IP protocol for POLDIP2 antibody 15080-1-AP	Download protocol
WB protocol for POLDIP2 antibody 15080-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	EMBO J Poldip2 promotes mtDNA elimination during Drosophila spermatogenesis to ensure maternal inheritance Authors - Ziming Wang View Article
mouse	WB	Proc Natl Acad Sci U S A DNA polymerase δ-interacting protein 2 is a processivity factor for DNA polymerase λ during 8-oxo-7,8-dihydroguanine bypass. Authors - Maga Giovanni G View Article
human	IF	Cell Cycle PDIP38 is translocated to the spliceosomes/nuclear speckles in response to UV-induced DNA damage and is required for UV-induced alternative splicing of MDM2. Authors - Agnes Wong View Article
mouse,human	WB	Cells Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA. Authors - Jana Key View Article
mouse,rat	WB	Life Sci Poldip2 Aggravates inflammation in diabetic retinopathy by impairing mitophagy via the AMPK/ULK1/Pink1 pathway Authors - Siyu Lin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15080-1-AP

WB analysis of HeLa using 15080-1-AP

WB analysis of HepG2 using 15080-1-AP

WB analysis of Jurkat using 15080-1-AP

IP experiment of HeLa using 15080-1-AP

IHC staining of human stomach cancer using 15080-1-AP

IHC staining of human stomach cancer using 15080-1-AP

IF Staining of HeLa using 15080-1-AP

POLDIP2 Polyclonal antibody

POLDIP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Poldip2 promotes mtDNA elimination during Drosophila spermatogenesis to ensure maternal inheritance

DNA polymerase δ-interacting protein 2 is a processivity factor for DNA polymerase λ during 8-oxo-7,8-dihydroguanine bypass.

PDIP38 is translocated to the spliceosomes/nuclear speckles in response to UV-induced DNA damage and is required for UV-induced alternative splicing of MDM2.

Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.

Poldip2 Aggravates inflammation in diabetic retinopathy by impairing mitophagy via the AMPK/ULK1/Pink1 pathway