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Target

Host

Species Reactivity

Species Reactivity

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Conjugate

  • Featured Product
  • KD/KO Validated

PNMA1 Polyclonal antibody

PNMA1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13631-1-AP

Synonyms

37 kDa neuronal protein, MA1, paraneoplastic antigen MA1, PNMA1

Validation Data Gallery

Applications:
  • WB analysis using 13631-1-AP

    WB analysis using 13631-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13631-1-AP (PNMA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 13631-1-AP

    IP experiment of mouse testis using 13631-1-AP

    IP result of anti-PNMA1 (IP:13631-1-AP, 4ug; Detection:13631-1-AP 1:500) with mouse testis tissue lysate 4000ug.

  • IHC staining of mouse brain using 13631-1-AP

    IHC staining of mouse brain using 13631-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13631-1-AP (PNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13631-1-AP

    IHC staining of mouse brain using 13631-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13631-1-AP (PNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 13631-1-AP

    IHC staining of mouse testis using 13631-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13631-1-AP (PNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 13631-1-AP

    IHC staining of mouse testis using 13631-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13631-1-AP (PNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 13631-1-AP

    IHC staining of human gliomas using 13631-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 13631-1-AP (PNMA1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human gliomas using 13631-1-AP

    IHC staining of human gliomas using 13631-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 13631-1-AP (PNMA1 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inPC-3 cells, SH-SY5Y cells, mouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse brain tissue, mouse testis tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13631-1-AP targets PNMA1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PNMA1 fusion protein Ag4549 相同性解析による交差性が予測される生物種
Full Name paraneoplastic antigen MA1
Calculated molecular weight 353 aa, 40 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC039577
Gene symbol PNMA1
Gene ID (NCBI) 9240
RRIDAB_2167615
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PNMA1 antibody 13631-1-APDownload protocol
IHC protocol for PNMA1 antibody 13631-1-APDownload protocol
IP protocol for PNMA1 antibody 13631-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomed Pharmacother

PNMA1, regulated by miR-33a-5p, promotes proliferation and EMT in hepatocellular carcinoma by activating the Wnt/β-catenin pathway.

Authors - Pengpeng Liu
  • KD Validated
View Article
humanWB,IHC

Int J Clin Exp Pathol

PNMA1 promotes cell growth in human pancreatic ductal adenocarcinoma.

Authors - Shu-Heng Jiang
  • KD Validated
View Article