Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 11027-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 11027-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 11027-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 11027-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11027-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 11027-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11027-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 11027-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 11027-1-AP
IP result of anti-PLOD3 (IP:11027-1-AP, 3ug; Detection:11027-1-AP 1:500) with HepG2 cells lysate 6000ug.
IHC staining of human pancreas cancer using 11027-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11027-1-AP (PLOD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 11027-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11027-1-AP (PLOD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 11027-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 11027-1-AP (PLOD3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 11027-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 11027-1-AP (PLOD3 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 11027-1-AP
Immunofluorescent analysis of HepG2 cells using 11027-1-AP (PLOD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
IF Staining of HepG2 using 11027-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PLOD3 antibody (11027-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

PLOD3 Polyclonal antibody

PLOD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11027-1-AP

Publications(36)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Galactosylhydroxylysine-glucosyltransferase, LH3, Lysyl hydroxylase 3, Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3, Procollagen galactosyltransferase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (36)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, PC-3 cells, HepG2 cells, HeLa cells, human placenta tissue, mouse placenta tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human pancreas cancer tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 26 publications below
IHC	See 7 publications below
IF	See 10 publications below
IP	See 1 publications below

Product Information

11027-1-AP targets PLOD3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, drosophila
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1480 Product name: Recombinant human PLOD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 339-738 aa of BC011674 Sequence: HEPHIADSWPQLQDHFSAVKLVGPEEALSPGEARDMAMDLCRQDPECEFYFSLDADAVLTNLQTLRILIEENRKVIAPMLSRHGKLWSNFWGALSPDEYYARSEDYVELVQRKRVGVWNVPYISQAYVIRGDTLRMELPQRDVFSGSDTDPDMAFCKSFRDKGIFLHLSNQHEFGRLLATSRYDTEHLHPDLWQIFDNPVDWKEQYIHENYSRALEGEGIVEQPCPDVYWFPLLSEQMCDELVAEMEHYGQWSGGRHEDSRLAGGYENVPTVDIHMKQVGYEDQWLQLLRTYVGPMTESLFPGYHTKARAVMNFVVRYRPDEQPSLRPHHDSSTFTLNVALNHKGLDYEGGGCRFLRYDCVISSPRKGWALLHPGRLTHYHEGLPTTWGTRYIMVSFVDP 相同性解析による交差性が予測される生物種
Full Name	procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
Calculated molecular weight	738 aa, 85 kDa
Observed molecular weight	80-85 kDa
GenBank accession number	BC011674
Gene Symbol	PLOD3
Gene ID (NCBI)	8985
RRID	AB_2165781
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O60568
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.

Protocols

Product Specific Protocols
IF protocol for PLOD3 antibody 11027-1-AP	Download protocol
IHC protocol for PLOD3 antibody 11027-1-AP	Download protocol
IP protocol for PLOD3 antibody 11027-1-AP	Download protocol
WB protocol for PLOD3 antibody 11027-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Sci Adv p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi. Authors - Xiaochao Tan View Article
		Nat Commun Molecular architecture of the multifunctional collagen lysyl hydroxylase and glycosyltransferase LH3. Authors - Luigi Scietti View Article
human	WB,IF	Nat Commun Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis. Authors - Blerida Banushi View Article
human	WB	Am J Hum Genet A connective tissue disorder caused by mutations of the lysyl hydroxylase 3 gene. Authors - Salo Antti M AM View Article
		Matrix Biol Antibodies and methods for immunohistochemistry of extracellular matrix proteins. Authors - Steffen Rickelt View Article
mouse	WB	PLoS Genet Abnormal type I collagen post-translational modification and crosslinking in a cyclophilin B KO mouse model of recessive osteogenesis imperfecta. Authors - Wayne A Cabral View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11027-1-AP

WB analysis using 11027-1-AP

WB analysis of PC-3 using 11027-1-AP

WB analysis of mouse placenta using 11027-1-AP

WB analysis of HeLa using 11027-1-AP

WB analysis of human placenta using 11027-1-AP

WB analysis of HepG2 using 11027-1-AP

WB analysis of A549 using 11027-1-AP

IP experiment of HepG2 using 11027-1-AP

IHC staining of human pancreas cancer using 11027-1-AP

IHC staining of human pancreas cancer using 11027-1-AP

IHC staining of human pancreas using 11027-1-AP

IHC staining of human pancreas using 11027-1-AP

IF Staining of HepG2 using 11027-1-AP

IF Staining of HepG2 using 11027-1-AP

PLOD3 Polyclonal antibody

PLOD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Molecular architecture of the multifunctional collagen lysyl hydroxylase and glycosyltransferase LH3.

Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis.

A connective tissue disorder caused by mutations of the lysyl hydroxylase 3 gene.

Antibodies and methods for immunohistochemistry of extracellular matrix proteins.

Abnormal type I collagen post-translational modification and crosslinking in a cyclophilin B KO mouse model of recessive osteogenesis imperfecta.