Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, A549 cells, HeLa cells, MDA-MB-453s cells, U2OS cells, Jurkat cells, mouse testis tissue, rat testis tissue |
| Positive IHC detected in | rat stomach tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
| Positive FC (Intra) detected in | Ramos cells |
PLK1 expression is low or nonexistent in normal tissues, but high in tumor tissues.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 7 publications below |
| WB | See 51 publications below |
| IHC | See 13 publications below |
| IF | See 9 publications below |
| IP | See 5 publications below |
| CoIP | See 3 publications below |
Product Information
10305-1-AP targets PLK1 in WB, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0284 Product name: Recombinant human PLK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 415-603 aa of BC002369 Sequence: VDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS 相同性解析による交差性が予測される生物種 |
| Full Name | polo-like kinase 1 (Drosophila) |
| Calculated molecular weight | 68 kDa |
| Observed molecular weight | 68 kDa |
| GenBank accession number | BC002369 |
| Gene Symbol | PLK1 |
| Gene ID (NCBI) | 5347 |
| RRID | AB_2877731 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P53350 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PLK1(Polo-like kinase 1), also named as PLK and STPK13, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and CDC5/Polo subfamily. PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.The localization of Polo changes during the cell cycle. In interphase, Polo shows a mostly diffuse cytoplasmic localization. Polo becomes concentrated on centrosomes from early prophase and appears on centromeres or kinetochores from late prophase, before nuclear envelope breakdown (NEB). After anaphase onset, Polo relocalizes to the central spindle and remains enriched at the midbody ring in late stages of cytokinesis (PMID: 29167465).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PLK1 antibody 10305-1-AP | Download protocol |
| IHC protocol for PLK1 antibody 10305-1-AP | Download protocol |
| WB protocol for PLK1 antibody 10305-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Med Translation control of the immune checkpoint in cancer and its therapeutic targeting. | ||
Nat Commun CRISPR screens reveal convergent targeting strategies against evolutionarily distinct chemoresistance in cancer | ||
Nat Commun Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer. | ||
Nat Commun Selective identification of epigenetic regulators at methylated genomic sites by SelectID | ||
J Adv Res Rhein suppresses lactate metabolism and melanoma progression via downregulating LDHA/H3K18 lactylation/PLK1 axis.
| ||
J Clin Invest PLK1 inhibition dampens NLRP3 inflammasome-elicited response in inflammatory disease models |