• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLA2G1B Monoclonal antibody

PLA2G1B Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F2C10

Cat no : 66397-1-Ig

Synonyms

Group IB phospholipase A2, Phospholipase A2, PLA2, PLA2A, PLA2G1B, PPLA2

Validation Data Gallery

Applications:
  • WB analysis of pig pancreas using 66397-1-Ig

    WB analysis of pig pancreas using 66397-1-Ig

    pig pancreas tissue were subjected to SDS PAGE followed by western blot with 66397-1-Ig (PLA2G1B Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 66397-1-Ig

    IHC staining of human pancreas using 66397-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66397-1-Ig (PLA2G1B antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas using 66397-1-Ig

    IHC staining of human pancreas using 66397-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66397-1-Ig (PLA2G1B antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inpig pancreas tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

66397-1-Ig targets PLA2G1B in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PLA2G1B fusion protein Ag8812 相同性解析による交差性が予測される生物種
Full Name phospholipase A2, group IB (pancreas)
Calculated molecular weight70aa,8 kDa; 148aa,16 kDa
Observed molecular weight 13-16 kDa
GenBank accession numberBC005386
Gene symbol PLA2G1B
Gene ID (NCBI) 5319
RRIDAB_2881771
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PLA2G1B antibody 66397-1-IgDownload protocol
IHC protocol for PLA2G1B antibody 66397-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols