Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of human testis using 12541-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 12541-1-AP (PIP5K1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12541-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12541-1-AP (PIP5K1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 12541-1-AP
IP result of anti-PIP5K1B (IP:12541-1-AP, 4ug; Detection:12541-1-AP 1:500) with mouse testis tissue lysate 4000ug.
IHC staining of human lung using 12541-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 12541-1-AP (PIP5K1B antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 12541-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 12541-1-AP (PIP5K1B antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human heart using 12541-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 12541-1-AP (PIP5K1B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 12541-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 12541-1-AP (PIP5K1B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 12541-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 12541-1-AP (PIP5K1B antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 12541-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 12541-1-AP (PIP5K1B antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung using 12541-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 12541-1-AP (PIP5K1B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 12541-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 12541-1-AP (PIP5K1B antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

PIP5K1B Polyclonal antibody

PIP5K1B Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12541-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

PIP5K1 beta, PIP5K1B, PIP5KIbeta, Protein STM 7, PtdIns(4)P 5 kinase 1 beta, STM7

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human testis tissue, human brain tissue
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human lung tissue, human heart tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below

Product Information

12541-1-AP targets PIP5K1B in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3261 Product name: Recombinant human PIP5K1B protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 107-405 aa of BC030587 Sequence: SEGSNLTPAHHYPDFRFKTYAPLAFRYFRELFGIKPDDYLYSICSEPLIELSNPGASGSLFFVTSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCMQSGGINIRIVVMNNVLPRSMRMHFTYDLKGSTYKRRASRKEREKSNPTFKDLDFLQDMHEGLYFDTETYNALMKTLQRDCRVLESFKIMDYSLLLGIHFLDHSLKEKEEETPQNVPDAKRTGMQKVLYSTAMESIQGPGKSGDGIITENPDTMGGIPAKSHRGEKLLLFTGIIDILQSYRLMKKLEHSW 相同性解析による交差性が予測される生物種
Full Name	phosphatidylinositol-4-phosphate 5-kinase, type I, beta
Calculated molecular weight	549 aa, 62 kDa
Observed molecular weight	62 kDa
GenBank accession number	BC030587
Gene Symbol	PIP5K1B
Gene ID (NCBI)	8395
RRID	AB_10734598
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O14986
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for PIP5K1B antibody 12541-1-AP	Download protocol
IP protocol for PIP5K1B antibody 12541-1-AP	Download protocol
WB protocol for PIP5K1B antibody 12541-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Clin Invest Spastic paraplegia proteins spastizin and spatacsin mediate autophagic lysosome reformation. Authors - Jaerak Chang View Article
mouse	WB	EMBO J PTPRN2 and PLCβ1 promote metastatic breast cancer cell migration through PI(4,5)P2-dependent actin remodeling. Authors - Caitlin A Sengelaub View Article
human	WB	EMBO J Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKHM1 membrane cycling during autophagosome-lysosome fusion. Authors - Takashi Baba KD Validated View Article
human	WB	Cell Chem Biol Targeting the PI5P4K Lipid Kinase Family in Cancer Using Covalent Inhibitors. Authors - Sindhu Carmen Sivakumaren View Article
		EMBO J Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKMH1 membrane cycling during autophagosome-lysosome fusion. Authors - Takashi Baba View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human testis using 12541-1-AP

WB analysis of human brain using 12541-1-AP

IP experiment of mouse testis using 12541-1-AP

IHC staining of human lung using 12541-1-AP

IHC staining of human lung using 12541-1-AP

IHC staining of human heart using 12541-1-AP

IHC staining of human heart using 12541-1-AP

IHC staining of human kidney using 12541-1-AP

IHC staining of human kidney using 12541-1-AP

IHC staining of human lung using 12541-1-AP

IHC staining of human lung using 12541-1-AP

PIP5K1B Polyclonal antibody

PIP5K1B Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Spastic paraplegia proteins spastizin and spatacsin mediate autophagic lysosome reformation.

PTPRN2 and PLCβ1 promote metastatic breast cancer cell migration through PI(4,5)P2-dependent actin remodeling.

Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKHM1 membrane cycling during autophagosome-lysosome fusion.

Targeting the PI5P4K Lipid Kinase Family in Cancer Using Covalent Inhibitors.

Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKMH1 membrane cycling during autophagosome-lysosome fusion.