Validation Data Gallery

Filter: AllWBIHCIF-P

WB analysis of HeLa using 23274-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 23274-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 23274-1-AP
Non-treated and CCCP treated HeLa cells were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 23274-1-AP
WB result of PINK1 antibody (23274-1-AP, 1:600) with si-Control and si-PINK1 transfected HeLa cells.
IHC staining of mouse brain using 23274-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23274-1-AP (PINK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 23274-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23274-1-AP (PINK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 23274-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 23274-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of rat brain using 23274-1-AP
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of rat brain using 23274-1-AP
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

PINK1 Polyclonal antibody

PINK1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 23274-1-AP

Publications(771)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF-P, IP, CoIP, ChIP, ELISA

Synonyms

BRPK, EC:2.7.11.1, PARK6, PTEN induced putative kinase 1, PTEN-induced putative kinase protein 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (771)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, CCCP treated HeLa cells, PC-12 cells
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue, rat brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 41 publications below
WB	See 639 publications below
IHC	See 65 publications below
IF	See 125 publications below
IP	See 4 publications below
CoIP	See 8 publications below
ChIP	See 1 publications below

Product Information

23274-1-AP targets PINK1 in WB, IHC, IF-P, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, rabbit, monkey, zebrafish, sheep, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag19825 Product name: Recombinant human PINK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 336-581 aa of BC028215 Sequence: PRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAAL 相同性解析による交差性が予測される生物種
Full Name	PTEN induced putative kinase 1
Calculated molecular weight	581 aa, 63 kDa
Observed molecular weight	65 kDa, 45 kDa
GenBank accession number	BC028215
Gene Symbol	PINK1
Gene ID (NCBI)	65018
RRID	AB_2879244
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	Q9BXM7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa forms (PMID: 18221368; 25108683; 18031932). The half-life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.

Protocols

Product Specific Protocols
IF protocol for PINK1 antibody 23274-1-AP	Download protocol
IHC protocol for PINK1 antibody 23274-1-AP	Download protocol
WB protocol for PINK1 antibody 23274-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Signal Transduct Target Ther A two-strata energy flux system driven by a stress hormone prioritizes cardiac energetics Authors - Zhiheng Rao View Article
mouse	WB	Cell Metab Excessive vigorous exercise impairs cognitive function through a muscle-derived mitochondrial pretender. Authors - Yan Huang View Article
human	WB,IF	Bioact Mater Copper doped bioactive glass promotes matrix vesicles-mediated biomineralization via osteoblast mitophagy and mitochondrial dynamics during bone regeneration Authors - Ziji Ling View Article
human	WB	Bioact Mater NIR-responsive bio-system with sequential antibacterial and immunomodulatory effects for the treatment of periodontitis Authors - Xinyi Qiu View Article
	WB	Bioact Mater Near infrared enhanced palladium loaded siraitia grosvenorii carbon dots amplify mitophagy for acute lung injury immunotherapy. Authors - Jing Zhang View Article
mouse	WB	Bioact Mater Astaxanthin-metformin conjugate nanomicelles-loaded hydrogel reprograms macrophage metabolism via mitochondrion-targeted regulation for diabetic wound healing. Authors - Xin-Nan Teng View Article

Validation Data Gallery

WB analysis of HeLa using 23274-1-AP

WB analysis using 23274-1-AP

WB analysis using 23274-1-AP

WB analysis of HeLa using 23274-1-AP

IHC staining of mouse brain using 23274-1-AP

IHC staining of mouse brain using 23274-1-AP

IF Staining of mouse brain using 23274-1-AP

IF Staining of mouse brain using 23274-1-AP

IF Staining of rat brain using 23274-1-AP

IF Staining of rat brain using 23274-1-AP

PINK1 Polyclonal antibody

PINK1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A two-strata energy flux system driven by a stress hormone prioritizes cardiac energetics

Excessive vigorous exercise impairs cognitive function through a muscle-derived mitochondrial pretender.

Copper doped bioactive glass promotes matrix vesicles-mediated biomineralization via osteoblast mitophagy and mitochondrial dynamics during bone regeneration

NIR-responsive bio-system with sequential antibacterial and immunomodulatory effects for the treatment of periodontitis

Near infrared enhanced palladium loaded siraitia grosvenorii carbon dots amplify mitophagy for acute lung injury immunotherapy.

Astaxanthin-metformin conjugate nanomicelles-loaded hydrogel reprograms macrophage metabolism via mitochondrion-targeted regulation for diabetic wound healing.

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