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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PI3 Kinase p110 Delta Monoclonal antibody

PI3 Kinase p110 Delta Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2G11C6

Cat no : 67964-1-Ig

Synonyms

p110D, PI3 kinase subunit delta, PI3K delta, PIK3CD, PtdIns 3 kinase subunit delta

Validation Data Gallery

Applications:
  • WB analysis of LNCaP using 67964-1-Ig

    WB analysis of LNCaP using 67964-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67964-1-Ig (PIK3CD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 67964-1-Ig

    WB analysis of Jurkat using 67964-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67964-1-Ig (PIK3CD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of LNCaP using 67964-1-Ig

    IF Staining of LNCaP using 67964-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using PIK3CD antibody (67964-1-Ig, Clone: 2G11C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, Jurkat cells
Positive IF detected inLNCaP cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

67964-1-Ig targets PI3 Kinase p110 Delta in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PI3 Kinase p110 Delta fusion protein Ag26995 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, catalytic, delta polypeptide
Calculated molecular weight 1044 aa, 120 kDa
Observed molecular weight110 kDa
GenBank accession numberBC132919
Gene symbol PIK3CD
Gene ID (NCBI) 5293
RRIDAB_2918715
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PI3 Kinase p110 Delta antibody 67964-1-IgDownload protocol
IF protocol for PI3 Kinase p110 Delta antibody 67964-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols