Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 22028-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22028-1-AP
WB result of PIK3C2A antibody (22028-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PIK3C2A transfected HeLa cells.
WB analysis of PC-3 using 22028-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22028-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 22028-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of PC-3 using 22028-1-AP
IP result of anti-PIK3C2A (IP:22028-1-AP, 4ug; Detection:22028-1-AP 1:15000) with PC-3 cells lysate 1480 ug.
IHC staining of mouse ovary using 22028-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse ovary using 22028-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 22028-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 22028-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse ovary using 22028-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 22028-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PIK3C2A antibody (22028-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

PIK3C2A Polyclonal antibody

PIK3C2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 22028-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:2.7.1.137, Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, Phosphoinositide 3-kinase-C2-alpha, PI3 K C2A, PI3K C2 alpha

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (11)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, PC-3 cells, human placenta tissue
Positive IP detected in	PC-3 cells
Positive IHC detected in	mouse ovary tissue, mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 10 publications below
IHC	See 1 publications below
IF	See 5 publications below

Product Information

22028-1-AP targets PIK3C2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16894 Product name: Recombinant human PIK3C2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-338 aa of BC113658 Sequence: MAQISSNSGFKECPSSHPEPTRAKDVDKEEALQMEAEALAKLQKDRQVTDNQRGFELSSSTRKKAQVYNKQDYDLMVFPESDSQKRALDIDVEKLTQAELEKLLLDDSFETKKTPVLPVTPILSPSFSAQLYFRPTIQRGQWPPGLPGPSTYALPSIYPSTYSKQAAFQNGFNPRMPTFPSTEPIYLSLPGQSPYFSYPLTPATPFHPQGSLPIYRPVVSTDMAKLFDKIASTSEFLKNGKARTDLEITDSKVSNLQVSPKSEDISKFDWLDLDPLSKPKVDNVEVLDHEEEKNVSSLLAKDPWDAVLLEERSTANCHLERKVNGKSLSVATVTRSQS 相同性解析による交差性が予測される生物種
Full Name	phosphoinositide-3-kinase, class 2, alpha polypeptide
Calculated molecular weight	1686 aa, 191 kDa
Observed molecular weight	191 kDa
GenBank accession number	BC113658
Gene Symbol	PIK3C2A
Gene ID (NCBI)	5286
RRID	AB_11183760
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O00443
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for PIK3C2A antibody 22028-1-AP	Download protocol
IHC protocol for PIK3C2A antibody 22028-1-AP	Download protocol
IP protocol for PIK3C2A antibody 22028-1-AP	Download protocol
WB protocol for PIK3C2A antibody 22028-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Science PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation. Authors - Federico Gulluni KD Validated View Article
human	WB,IF	Cancer Cell Mitotic Spindle Assembly and Genomic Stability in Breast Cancer Require PI3K-C2α Scaffolding Function. Authors - Federico Gulluni View Article
rat	WB,IF	Cell Res Phosphatidylinositol 3,4-bisphosphate regulates neurite initiation and dendrite morphogenesis via actin aggregation. Authors - Shu-Xin Zhang View Article
human	WB,IF	J Cell Sci Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization Authors - Ellis L Ryan KD Validated View Article
human	WB,IF	J Cell Sci Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization. Authors - Ellis L Ryan View Article
human	WB	Front Cell Infect Microbiol The Role of Tim-3 on dNK Cells Dysfunction During Abnormal Pregnancy With Toxoplasma gondii Infection. Authors - Teng Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 22028-1-AP

WB analysis of HeLa using 22028-1-AP

WB analysis of PC-3 using 22028-1-AP

WB analysis of HeLa using 22028-1-AP

WB analysis of human placenta using 22028-1-AP

IP experiment of PC-3 using 22028-1-AP

IHC staining of mouse ovary using 22028-1-AP

IHC staining of mouse ovary using 22028-1-AP

IHC staining of mouse heart using 22028-1-AP

IHC staining of mouse heart using 22028-1-AP

IHC staining of mouse ovary using 22028-1-AP

IF Staining of HeLa using 22028-1-AP

PIK3C2A Polyclonal antibody

PIK3C2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation.

Mitotic Spindle Assembly and Genomic Stability in Breast Cancer Require PI3K-C2α Scaffolding Function.

Phosphatidylinositol 3,4-bisphosphate regulates neurite initiation and dendrite morphogenesis via actin aggregation.

Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization

Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization.

The Role of Tim-3 on dNK Cells Dysfunction During Abnormal Pregnancy With Toxoplasma gondii Infection.