PI4KB Polyclonal antibody

PI4KB Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13247-1-AP

Synonyms

PI4 kinase beta, PI4K BETA, pi4K92, PI4KB, PI4Kbeta, PI4KIIIbeta, PIK4CB, PtdIns 4 kinase beta



Tested Applications

Positive WB detected inK-562 cells, NIH-3T3 cells (RNAi)
Positive IP detected inK-562 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13247-1-AP targets PI4KB in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PI4KB fusion protein Ag3989 相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol 4-kinase, catalytic, beta
Calculated molecular weight 801 aa, 90 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC040300
Gene symbol PI4KB
Gene ID (NCBI) 5298
RRIDAB_2252370
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PI4KB antibody 13247-1-APDownload protocol
IHC protocol for PI4KB antibody 13247-1-APDownload protocol
IP protocol for PI4KB antibody 13247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Cell Res

Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity

Authors - Ying Gao
humanIF

EMBO J

Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB.

Authors - Zhengming Wu
humanWB

PLoS Pathog

Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.

Authors - Tao Wang
human

Virol Sin

Construction of coxsackievirus B5 viruses with luciferase reporters and their applications in vitro and in vivo

Authors - Shangrui Guo
ratWB

Cereb Cortex

Neonatal exposure to sevoflurane induces adolescent neurobehavioral dysfunction by interfering with hippocampal glycerophoslipid metabolism in rats.

Authors - Yonghai Wang

J Cell Sci

Depletion of phosphatidylinositol 4-phosphate at the Golgi translocates K-Ras to mitochondria.

Authors - Taylor E Miller