Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of K-562 using 13247-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 13247-1-AP (PI4KB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of NIH-3T3 cells (RNAi) using 13247-1-AP
WB result of anti- PI4Kb (13247-1-AP) with NIH-3T3 cells (RNAi).
WB analysis of Jurkat using 13247-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13247-1-AP (PI4KB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 13247-1-AP
IP result of anti-PI4KB (IP:13247-1-AP, 4ug; Detection:13247-1-AP 1:300) with K-562 cells lysate 3600ug.
IHC staining of human liver using 13247-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13247-1-AP (PI4KB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 13247-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13247-1-AP (PI4KB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of C6 using 13247-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using PI4KB antibody (13247-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

PI4KB Polyclonal antibody

PI4KB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13247-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PtdIns 4-kinase beta, PIK4CB, PI4KIII, PI4K-beta, Phosphatidylinositol 4-kinase beta

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	K-562 cells, NIH-3T3 cells (RNAi)
Positive IP detected in	K-562 cells
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	C6 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 10 publications below
IF	See 2 publications below

Product Information

13247-1-AP targets PI4KB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3989 Product name: Recombinant human PI4KB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 449-801 aa of BC040300 Sequence: IGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQLAHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKKQSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSPRNLGFETSAFKLTTEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHGSSTIRNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGIM 相同性解析による交差性が予測される生物種
Full Name	phosphatidylinositol 4-kinase, catalytic, beta
Calculated molecular weight	801 aa, 90 kDa
Observed molecular weight	100 kDa
GenBank accession number	BC040300
Gene Symbol	PI4KB
Gene ID (NCBI)	5298
RRID	AB_2252370
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UBF8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PI4KB(phosphatidylinositol 4-kinase beta) is also named as PIK4CB, NPIK, PI4K92 and belongs to the PI3/PI4-kinase family. It may regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation and involved in Golgi-to-plasma membrane trafficking. PI4KB is a type III enzyme that is sensitive to wortmannin(PMID:9020160). This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for PI4KB antibody 13247-1-AP	Download protocol
IHC protocol for PI4KB antibody 13247-1-AP	Download protocol
IP protocol for PI4KB antibody 13247-1-AP	Download protocol
WB protocol for PI4KB antibody 13247-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	Cell Res Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity Authors - Ying Gao View Article
human	IF	EMBO J Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB. Authors - Zhengming Wu View Article
human	WB	PLoS Pathog Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication. Authors - Tao Wang View Article
human		Virol Sin Construction of coxsackievirus B5 viruses with luciferase reporters and their applications in vitro and in vivo Authors - Shangrui Guo View Article
human	WB,IF	J Virol N-terminal acetyltransferase 6 facilitates enterovirus 71 replication by regulating PI4KB expression and replication organelle biogenesis Authors - Hang Yang View Article
mouse	WB	J Neurosci PI4KIIIβ-Mediated Phosphoinositides Metabolism Regulates Function of the VTA Dopaminergic Neurons and Depression-Like Behavior Authors - Yuqi Sang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of K-562 using 13247-1-AP

WB analysis of NIH-3T3 cells (RNAi) using 13247-1-AP

WB analysis of Jurkat using 13247-1-AP

IP experiment of K-562 using 13247-1-AP

IHC staining of human liver using 13247-1-AP

IHC staining of human liver using 13247-1-AP

IF Staining of C6 using 13247-1-AP

PI4KB Polyclonal antibody

PI4KB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity

Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB.

Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.

Construction of coxsackievirus B5 viruses with luciferase reporters and their applications in vitro and in vivo

N-terminal acetyltransferase 6 facilitates enterovirus 71 replication by regulating PI4KB expression and replication organelle biogenesis

PI4KIIIβ-Mediated Phosphoinositides Metabolism Regulates Function of the VTA Dopaminergic Neurons and Depression-Like Behavior