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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated PI3 Kinase p110 Alpha Monoclonal antibody

PI3 Kinase p110 Alpha Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F6A7

Cat no : CL594-67071

Synonyms

p110 alpha, PI3 Kinase p110 Alpha, PI3 kinase subunit alpha, PI3K, PI3K alpha, PIK3CA, PtdIns 3 kinase subunit alpha

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL594-67071

    IF Staining of HeLa using CL594-67071

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 PI3 Kinase p110 Alpha antibody (CL594-67071, Clone: 1F6A7 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL594-67071

    FC experiment of Jurkat using CL594-67071

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PI3 Kinase p110 Alpha (CL594-67071, Clone:1F6A7) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 67071-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-67071 targets PI3 Kinase p110 Alpha in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PI3 Kinase p110 Alpha fusion protein Ag17392 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, catalytic, alpha polypeptide
Calculated molecular weight 1068 aa, 124 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC113601
Gene symbol PIK3CA
Gene ID (NCBI) 5290
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).

Protocols

Product Specific Protocols
IF protocol for CL594 PI3 Kinase p110 Alpha antibody CL594-67071Download protocol
FC protocol for CL594 PI3 Kinase p110 Alpha antibody CL594-67071Download protocol
Standard Protocols
Click here to view our Standard Protocols