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- KD/KO Validated
PHF19 Polyclonal antibody
PHF19 Polyclonal Antibody for IF, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11895-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | rat brain tissue |
| Positive IF detected in | Mouse ES cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
Product Information
11895-1-AP targets PHF19 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PHF19 fusion protein Ag2511 相同性解析による交差性が予測される生物種 |
| Full Name | PHD finger protein 19 |
| Calculated molecular weight | 580 aa, 63 kDa |
| Observed molecular weight | 66 kDa |
| GenBank accession number | BC022374 |
| Gene symbol | PHF19 |
| Gene ID (NCBI) | 26147 |
| RRID | AB_10804890 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PHD FINGER PROTEIN 19(PHF19), a protein homologous to the Drosophila 'Polycomblike' (Pcl) protein, encoded by human gene PCL, which encodes 2 deduced proteins: a short form of 207 aa and a long form of 580 aa. The PCL family proteins contains a PCL homology domain, a Tudor domain, 2 PHD fingers and a C-terminal tail. It is highly expressed in thymus and heart, while 508aa form predominant in placenta, skeletal muscle and kidney and 207aa form in liver and peripheral blood leukocytes. Both of these forms can inhibit transcriptional activity. And the long form is corelated with cancer.Acts as a transcritpional repressor. The mRNA level of PHF19 is low in many normal tissue, it's elevated in cancer-derived cell lines.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PHF19 antibody 11895-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell An H3K36 methylation-engaging Tudor motif of polycomb-like proteins mediates PRC2 complex targeting.
| ||
PLoS Genet Alu Elements in ANRIL Non-Coding RNA at Chromosome 9p21 Modulate Atherogenic Cell Functions through Trans-Regulation of Gene Networks. | ||
PLoS Genet Polycomb-like 3 promotes polycomb repressive complex 2 binding to CpG islands and embryonic stem cell self-renewal.
| ||
Front Immunol Comprehensive Pan-Cancer Genomic Analysis Reveals PHF19 as a Carcinogenic Indicator Related to Immune Infiltration and Prognosis of Hepatocellular Carcinoma. | ||
J Cancer ZNF750 exerted its Antitumor Action in Oral Squamous Cell Carcinoma by regulating E2F2. | ||
Scand J Gastroenterol Downregulation of PHF19 inhibits cell growth and migration in gastric cancer.
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