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Species Reactivity

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  • Featured Product
  • KD/KO Validated

Prohibitin Polyclonal antibody

Prohibitin Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10787-1-AP

Synonyms

Cell and organelle markers, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, PHB, PHB1, prohibitin

Validation Data Gallery

Applications:
  • WB analysis using 10787-1-AP

    WB analysis using 10787-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10787-1-AP (Prohibitin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 10787-1-AP

    WB analysis of HeLa using 10787-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of Raji using 10787-1-AP

    WB analysis of Raji using 10787-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 10787-1-AP

    WB analysis of A431 using 10787-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human liver using 10787-1-AP

    WB analysis of human liver using 10787-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 10787-1-AP

    WB analysis of human brain using 10787-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 10787-1-AP

    WB analysis of NIH/3T3 using 10787-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 10787-1-AP

    IHC staining of human liver cancer using 10787-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10787-1-AP (Prohibitin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 10787-1-AP

    IHC staining of human liver cancer using 10787-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10787-1-AP (Prohibitin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human heart using 10787-1-AP

    IHC staining of human heart using 10787-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 10787-1-AP (PHB antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 10787-1-AP

    IHC staining of human heart using 10787-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 10787-1-AP (PHB antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 10787-1-AP

    IF Staining of HepG2 using 10787-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10787-1-AP (Prohibitin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 10787-1-AP

    IF Staining of HeLa using 10787-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Prohibitin antibody (10787-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of H9C2 using 10787-1-AP

    IF Staining of H9C2 using 10787-1-AP

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using Prohibitin antibody (10787-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells, human liver tissue, human brain tissue, NIH/3T3 cells, HeLa cells, Raji cells, Jurkat cells, mouse brain tissue, mouse heart tissue, mouse kidney tissue, rat brain tissue, rat heart tissue, rat kidney tissue
Positive IHC detected inhuman liver cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inH9C2 cells, HepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

10787-1-AP targets Prohibitin in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Prohibitin fusion protein Ag1240 相同性解析による交差性が予測される生物種
Full Name prohibitin
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC013401
Gene symbol PHB
Gene ID (NCBI) 5245
RRIDAB_2164476
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis (PMID: 20840588 ). This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription (PMID: 18558096). Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis (PMID: 24402549).

Protocols

Product Specific Protocols
WB protocol for Prohibitin antibody 10787-1-APDownload protocol
IHC protocol for Prohibitin antibody 10787-1-APDownload protocol
IF protocol for Prohibitin antibody 10787-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Cholesterol Transport through Lysosome-Peroxisome Membrane Contacts.

Authors - Bei-Bei Chu
View Article
humanWB

Autophagy

PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis.

Authors - Chaojun Yan
View Article
mouseWB

Cell Death Differ

Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.

Authors - H Li
View Article
humanWB

Hum Mol Genet

Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.

Authors - McCray Brett A BA
View Article
mouseWB

Cell Mol Gastroenterol Hepatol

DJ-1 deficiency in hepatocytes improves liver ischemia-reperfusion injury by enhancing mitophagy.

Authors - Min Xu
View Article
humanWB

Elife

Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors.

Authors - Sven Dennerlein
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tom (Verified Customer) (02-24-2021)

HEK293T whole cell lysate. Membrane was blocked in 5% BSA for 1 hour then incubated overnight at 4 degrees with the primary antibody (1:1000)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
Prohibitin Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:10787-1-AP)