Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 12990-1-AP
WB result of PGRMC1 antibody (12990-1-AP; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PGRMC1 transfected HEK-293 cells.
WB analysis using 12990-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12990-1-AP (PGRMC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 12990-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12990-1-AP (PGRMC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12990-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12990-1-AP (PGRMC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 12990-1-AP
IP result of anti-PGRMC1 (IP:12990-1-AP, 3ug; Detection:12990-1-AP 1:1000) with MCF-7 cells lysate 1600ug.
IHC staining of human cervical cancer using 12990-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 12990-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse ovary using 12990-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse ovary using 12990-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 12990-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 12990-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12990-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 12990-1-AP (PGRMC1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung cancer using 12990-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 12990-1-AP (PGRMC1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary tumor using 12990-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12990-1-AP (PGRMC1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary tumor using 12990-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12990-1-AP (PGRMC1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human cervical cancer using 12990-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12990-1-AP (PGRMC1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cervical cancer using 12990-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12990-1-AP (PGRMC1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HEK-293 using 12990-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PGRMC1 antibody (12990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

PGRMC1 Polyclonal antibody

PGRMC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12990-1-AP

Publications(22)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PGRMC, mPR, Membrane-associated progesterone receptor component 1, HPR6.6

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (22)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse liver tissue, HepG2 cells, MCF-7 cells, mouse kidney tissue, mouse ovary tissue
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human cervical cancer tissue, human lung cancer tissue, human ovary tumor tissue, mouse kidney tissue, mouse ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 19 publications below
IHC	See 4 publications below
IF	See 3 publications below

Product Information

12990-1-AP targets PGRMC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3643 Product name: Recombinant human PGRMC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 43-195 aa of BC034238 Sequence: YKIVRGDQPAASGDSDDDEPPPLPRLKRRDFTPAELRRFDGVQDPRILMAINGKVFDVTKGRKFYGPEGPYGVFAGRDASRGLATFCLDKEALKDEYDDLSDLTAAQQETLSDWESQFTFKYHHVGKLLKEGEEPTVYSDEEEPKDESARKND 相同性解析による交差性が予測される生物種
Full Name	progesterone receptor membrane component 1
Calculated molecular weight	195 aa, 22 kDa
Observed molecular weight	22-28 kDa
GenBank accession number	BC034238
Gene Symbol	PGRMC1
Gene ID (NCBI)	10857
RRID	AB_2164342
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O00264
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Progesterone receptor membrane component 1 (PGRMC1) is a member of a multi-protein progesterone-binding complex. However, PGRMC1 shares homology with cytochrome b5-related proteins rather than hormone receptors (PMID: 18992768). It is a heme binding protein with biding sites for Src homology (SH2) and SH3 domain-containing proteins (PMID: 17583495). PGRMC1 is overexpressed in a variety of cancers, and thus represents an important biomarker for cancer progression and a potential target for anticancer drugs (PMID: 21730960). In nonmalignant tissues, PGRMC1 is highly expressed in the liver and kidney (PMID: 9705155; 20164297).

Protocols

Product Specific Protocols
IF protocol for PGRMC1 antibody 12990-1-AP	Download protocol
IHC protocol for PGRMC1 antibody 12990-1-AP	Download protocol
IP protocol for PGRMC1 antibody 12990-1-AP	Download protocol
WB protocol for PGRMC1 antibody 12990-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	EBioMedicine The Sigma-2 Receptor and Progesterone Receptor Membrane Component 1 are Different Binding Sites Derived From Independent Genes. Authors - Uyen B Chu KO Validated View Article
human	IHC	Int J Cancer Elevated progesterone receptor membrane component 1/sigma-2 receptor levels in lung tumors and plasma from lung cancer patients. Authors - Mir Shakeel U R SU View Article
rat	WB	Transl Psychiatry A potential mechanism underlying atypical antipsychotics-induced lipid disturbances. Authors - H L Cai View Article
rat	WB	Front Pharmacol A Potential Mechanism Underlying the Therapeutic Effects of Progesterone and Allopregnanolone on Ketamine-Induced Cognitive Deficits. Authors - Ting Cao View Article
human	WB	Biol Reprod Hyaluronic Acid Promotes the Expression of Progesterone Receptor Membrane Component 1 via Epigenetic Silencing of miR-139-5p in Granulosa Cells. Authors - Guangfeng Zhao View Article
human	WB	J Cell Sci Topology surveillance of the lanosterol demethylase CYP51A1 by Signal Peptide Peptidase Authors - Nikita Sergejevs View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 12990-1-AP

WB analysis using 12990-1-AP

WB analysis of mouse liver using 12990-1-AP

WB analysis of HepG2 using 12990-1-AP

IP experiment of MCF-7 using 12990-1-AP

IHC staining of human cervical cancer using 12990-1-AP

IHC staining of human cervical cancer using 12990-1-AP

IHC staining of mouse ovary using 12990-1-AP

IHC staining of mouse ovary using 12990-1-AP

IHC staining of mouse kidney using 12990-1-AP

IHC staining of mouse kidney using 12990-1-AP

IHC staining of human lung cancer using 12990-1-AP

IHC staining of human lung cancer using 12990-1-AP

IHC staining of human ovary tumor using 12990-1-AP

IHC staining of human ovary tumor using 12990-1-AP

IHC staining of human cervical cancer using 12990-1-AP

IHC staining of human cervical cancer using 12990-1-AP

IF Staining of HEK-293 using 12990-1-AP

PGRMC1 Polyclonal antibody

PGRMC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The Sigma-2 Receptor and Progesterone Receptor Membrane Component 1 are Different Binding Sites Derived From Independent Genes.

Elevated progesterone receptor membrane component 1/sigma-2 receptor levels in lung tumors and plasma from lung cancer patients.

A potential mechanism underlying atypical antipsychotics-induced lipid disturbances.

A Potential Mechanism Underlying the Therapeutic Effects of Progesterone and Allopregnanolone on Ketamine-Induced Cognitive Deficits.

Hyaluronic Acid Promotes the Expression of Progesterone Receptor Membrane Component 1 via Epigenetic Silencing of miR-139-5p in Granulosa Cells.

Topology surveillance of the lanosterol demethylase CYP51A1 by Signal Peptide Peptidase