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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PFKL Monoclonal antibody

PFKL Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H2G2

Cat no : 68385-1-Ig

Synonyms

DKFZp686G1648, DKFZp686L2097, FLJ30173, FLJ40909, PFK B, PFKL, Phosphofructokinase 1, phosphofructokinase, liver, Phosphohexokinase

Validation Data Gallery

Applications:
  • WB analysis using 68385-1-Ig

    WB analysis using 68385-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68385-1-Ig (PFKL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IHC staining of human lung cancer using 68385-1-Ig

    IHC staining of human lung cancer using 68385-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PFKL antibody included
  • IHC staining of human lung cancer using 68385-1-Ig

    IHC staining of human lung cancer using 68385-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PFKL antibody included
  • IHC staining of mouse liver using 68385-1-Ig

    IHC staining of mouse liver using 68385-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PFKL antibody included
  • IHC staining of mouse liver using 68385-1-Ig

    IHC staining of mouse liver using 68385-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PFKL antibody included
  • IHC staining of human ovary tumor using 68385-1-Ig

    IHC staining of human ovary tumor using 68385-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PFKL antibody included
  • IHC staining of human ovary tumor using 68385-1-Ig

    IHC staining of human ovary tumor using 68385-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PFKL antibody included
  • IHC staining of human liver using 68385-1-Ig

    IHC staining of human liver using 68385-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PFKL antibody included
  • IHC staining of human liver using 68385-1-Ig

    IHC staining of human liver using 68385-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PFKL antibody included
  • IF Staining of HuH-7 using 68385-1-Ig

    IF Staining of HuH-7 using 68385-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using PFKL antibody (68385-1-Ig, Clone: 1H2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HuH-7 using 68385-1-Ig

    IF Staining of HuH-7 using 68385-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HuH-7 cells using PFKL antibody (68385-1-Ig, Clone: 1H2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman lung cancer tissue, human liver tissue, human ovary tumor tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHuH-7 cells
Planning an IHC experiment? We recommend our IHCeasy PFKL Ready-To-Use IHC Kit. PFKL primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

68385-1-Ig targets PFKL in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PFKL fusion protein Ag8250 相同性解析による交差性が予測される生物種
Full Name phosphofructokinase, liver
Calculated molecular weight 85 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC008964
Gene symbol PFKL
Gene ID (NCBI) 5211
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PFKL (6-phosphofructokinase, liver type) is also named as PFK-B and belongs to the phosphofructokinase family. The PFKL gene encodes the liver isoform of phosphofructokinase (PFK) (ATP: D-fructose-6-phosphate-1-phosphotransferase). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. In human beings, PFK exists as a system of three subunits: these are the muscle(PFKM), liver(PFKL), and platelet(PFKP) PFKs (PMID: 2139864). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PFKL antibody 68385-1-IgDownload protocol
IHC protocol for PFKL antibody 68385-1-IgDownload protocol
IF protocol for PFKL antibody 68385-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols