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  • KD/KO Validated

PFKFB3 Polyclonal antibody

PFKFB3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13763-1-AP

Synonyms

6PF 2 K/Fru 2,6 P2ase 3, IPFK2, PFK/FBPase 3, PFK2, PFKFB3, PFKFB3-Specific



Tested Applications

Positive WB detected inHEK-293 cells, mouse thymus tissue, mouse heart tissue, A431 cells, HeLa cells, Jurkat cells, mouse spleen tissue
Positive IP detected inHEK-293 cells, mouse spleen tissue
Positive IHC detected inhuman liver cancer tissue, human kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13763-1-AP targets PFKFB3 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PFKFB3 fusion protein Ag4744 相同性解析による交差性が予測される生物種
Full Name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Calculated molecular weight 520 aa, 60 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC040482
Gene symbol PFKFB3
Gene ID (NCBI) 5209
RRIDAB_2162854
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PFKFB3, also named as NY-REN-56 and iPFK-2, plays a role in glucose metabolism. Its synthesis and degradation of fructose 2,6-bisphosphate. Endogenously generated adenosine cooperates with bacterial components to increase PFKFB3 isozyme activity, resulting in greater fructose 2,6-bisphosphate accumulation. PFKFB3 is required for increased growth, metabolic activity and is regulated through active JAK2 and STAT5.

Protocols

Product Specific Protocols
WB protocol for PFKFB3 antibody 13763-1-APDownload protocol
IHC protocol for PFKFB3 antibody 13763-1-APDownload protocol
IF protocol for PFKFB3 antibody 13763-1-APDownload protocol
IP protocol for PFKFB3 antibody 13763-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

FGF-dependent metabolic control of vascular development.

Authors - Pengchun Yu
humanWB

Cell

Role of PFKFB3-Driven Glycolysis in Vessel Sprouting.

Authors - De Bock Katrien K
humanWB

Cell Metab

CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.

Authors - Qidong Li
humanWB,IP

Cell Metab

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Authors - Mi Kyung Park
mouseWB

Circulation

Exercise-Induced Changes in Glucose Metabolism Promote Physiologic Cardiac Growth.

Authors - Andrew A Gibb
mouseWB

ACS Nano

Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy.

Authors - Mengshi Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Balawant (Verified Customer) (08-24-2022)

this antibody is working good for westerner blot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: colon tissue and colon cancer cell line
FH

Balawant (Verified Customer) (05-09-2022)

tis antibody is working great at 1:2000 diluton. I have used this antibody from total cells lysate of colonic epithelial cells form human origin.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Colonic epithelial cells
FH

Iram (Verified Customer) (04-13-2022)

Very good signal

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Colon cancer cells
FH

Susmita (Verified Customer) (03-11-2022)

This antibody works great for me.

  • Applications: Western Blot
  • Primary Antibody Dilution: WB: 1: 2000
  • Cell Tissue Type: PC cell line
FH

Hsin-Sheng (Verified Customer) (08-19-2019)

Strong bend intensity at around 60 kD. However, there is a non-specific band near 65-70 kD.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1;200
  • Cell Tissue Type: H1299