Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 12218-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12218-1-AP
WB result of PEPD antibody (12218-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEPD transfected HEK-293 cells.
WB analysis of HEK-293 using 12218-1-AP
WB result of PEPD antibody (12218-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEPD transfected HEK-293 cells.
WB analysis of MDA-MB-453s using 12218-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skin using 12218-1-AP
mouse skin tissue were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 12218-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12218-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12218-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 12218-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 12218-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 12218-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 12218-1-AP (PEPD antibody) at dilution of 1:50 (under 10x lens).
IF Staining of A549 using 12218-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PEPD antibody (12218-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

PEPD Polyclonal antibody

PEPD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12218-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:3.4.13.9, PRD, PROLIDASE, Xaa-Pro dipeptidase, X-Pro dipeptidase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, MDA-MB-453s cells, mouse skin tissue, HeLa cells, HepG2 cells, SH-SY5Y cells
Positive IHC detected in	human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:100-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 2 publications below

Product Information

12218-1-AP targets PEPD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2842 Product name: Recombinant human PEPD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 192-439 aa of BC015027 Sequence: LEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREV 相同性解析による交差性が予測される生物種
Full Name	peptidase D
Calculated molecular weight	493 aa, 55 kDa
Observed molecular weight	55 kDa
GenBank accession number	BC015027
Gene Symbol	PEPD
Gene ID (NCBI)	5184
RRID	AB_2161517
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P12955
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PEPD, also named as PRD, Prolidase, X-Pro dipeptidase, Imidodipeptidase, Peptidase D and Proline dipeptidase, belongs to the peptidase M24B family and Eukaryotic-type prolidase subfamily. PEPD splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. It plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). PEPD is considered as the most promising candidate genes for altering AAA risk, based on gene function, association evidence, gene expression, and protein expression.(PMID:21247474)

Protocols

Product Specific Protocols
IF protocol for PEPD antibody 12218-1-AP	Download protocol
IHC protocol for PEPD antibody 12218-1-AP	Download protocol
WB protocol for PEPD antibody 12218-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	BMC Med Genet Analysis of positional candidate genes in the AAA1 susceptibility locus for abdominal aortic aneurysms on chromosome 19. Authors - Lillvis John H JH View Article
human	IHC	Arch Med Res Relationship Between Echocardiographically Evaluated Aortic Stiffness and Prolidase Activity in Aortic Tissue of Patients with Critical Coronary Artery Disease. Authors - Aydemir Koçarslan View Article
human	WB	Nat Commun Parental experiences orchestrate locust egg hatching synchrony by regulating nuclear export of precursor miRNA Authors - Ya Nan Zhu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12218-1-AP

WB analysis of HEK-293 using 12218-1-AP

WB analysis of HEK-293 using 12218-1-AP

WB analysis of MDA-MB-453s using 12218-1-AP

WB analysis of mouse skin using 12218-1-AP

WB analysis using 12218-1-AP

WB analysis of HeLa using 12218-1-AP

WB analysis of HepG2 using 12218-1-AP

WB analysis using 12218-1-AP

WB analysis using 12218-1-AP

IHC staining of human lung cancer using 12218-1-AP

IF Staining of A549 using 12218-1-AP

PEPD Polyclonal antibody

PEPD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Analysis of positional candidate genes in the AAA1 susceptibility locus for abdominal aortic aneurysms on chromosome 19.

Relationship Between Echocardiographically Evaluated Aortic Stiffness and Prolidase Activity in Aortic Tissue of Patients with Critical Coronary Artery Disease.

Parental experiences orchestrate locust egg hatching synchrony by regulating nuclear export of precursor miRNA