Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse kidney tissue, HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IHC | See 5 publications below |
| IF | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
10507-2-AP targets PDZK1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PDZK1 fusion protein Ag0787 相同性解析による交差性が予測される生物種 |
| Full Name | PDZ domain containing 1 |
| Calculated molecular weight | 57 kDa |
| Observed molecular weight | 63-70 kDa |
| GenBank accession number | BC006496 |
| Gene Symbol | PDZK1 |
| Gene ID (NCBI) | 5174 |
| RRID | AB_2299495 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T2W1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PDZK1 is a PDZ domain-containing membrane scaffold protein widely expressed in a variety of epithelial cells. It plays a key role in cell signal transduction and ion channel regulation by interacting with a variety of ion channels, transporters, and receptors. PDZK1 simultaneously anchors multiple membrane proteins through its PDZ domain to regulate membrane expression and activity. PDZK1 maintains cholesterol homeostasis and participates in the regulation of the urate/anion exchanger (URAT1) in renal tubular uric acid reabsorption.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDZK1 antibody 10507-2-AP | Download protocol |
| IP protocol for PDZK1 antibody 10507-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncotarget Downregulation of vimentin expression increased drug resistance in ovarian cancer cells. | ||
Mol Med PDZK1 Is a Novel Factor in Breast Cancer That Is Indirectly Regulated by Estrogen through IGF-1R and Promotes Estrogen-Mediated Growth. | ||
Mol Med Correlation between PDZK1, Cdc37, Akt and breast cancer malignancy: the role of PDZK1 in cell growth through Akt stabilization by increasing and interacting with Cdc37. | ||
Cell Div miR-101-3p-mediated role of PDZK1 in hepatocellular carcinoma progression and the underlying PI3K/Akt signaling mechanism
| ||
Aging (Albany NY) Promoter hypomethylated PDZK1 acts as a tumorigenic gene in glioma by interacting with AKT1
| ||
Bone Res PDZK1 protects against mechanical overload-induced chondrocyte senescence and osteoarthritis by targeting mitochondrial function |