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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDXDC1 Polyclonal antibody

PDXDC1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21021-1-AP

Synonyms

KIAA0251, LP8165, PDXDC1

Validation Data Gallery

Applications:
  • WB analysis using 21021-1-AP

    WB analysis using 21021-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21021-1-AP (PDXDC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 21021-1-AP

    WB analysis using 21021-1-AP

    mouse testis lysates were subjected to SDS PAGE followed by western blot with 21021-1-AP (PDXDC1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 21021-1-AP

    IP experiment of HepG2 using 21021-1-AP

    IP result of anti-PDXDC1 (IP:21021-1-AP, 4ug; Detection:21021-1-AP 1:500) with HepG2 cells lysate 1680 ug.

  • IHC staining of human hepatocirrhosis using 21021-1-AP

    IHC staining of human hepatocirrhosis using 21021-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21021-1-AP (PDXDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human hepatocirrhosis using 21021-1-AP

    IHC staining of human hepatocirrhosis using 21021-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21021-1-AP (PDXDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 21021-1-AP

    IHC staining of human testis using 21021-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21021-1-AP (PDXDC1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 21021-1-AP

    IHC staining of human testis using 21021-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21021-1-AP (PDXDC1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 21021-1-AP

    IF Staining of HepG2 using 21021-1-AP

    Immunofluorescent analysis of HepG2 cells, using PDXDC1 antibody 21021-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IF Staining of HeLa using 21021-1-AP

    IF Staining of HeLa using 21021-1-AP

    Immunofluorescent analysis of Hela cells, using PDXDC1 antibody 21021-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHEK-293 cells, mouse testis tissue, HeLa cells, SGC-7901 cells, rat testis tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman hepatocirrhosis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21021-1-AP is PDXDC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDXDC1 fusion protein Ag15283 相同性解析による交差性が予測される生物種
Full Name pyridoxal-dependent decarboxylase domain containing 1
Calculated molecular weight 788 aa, 87 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC033748
Gene symbol PDXDC1
Gene ID (NCBI) 23042
RRIDAB_10695768
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PDXDC1 antibody 21021-1-APDownload protocol
IHC protocol for PDXDC1 antibody 21021-1-APDownload protocol
IF protocol for PDXDC1 antibody 21021-1-APDownload protocol
IP protocol for PDXDC1 antibody 21021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

J Mol Med (Berl)

Identification of PDXDC1 as a novel pleiotropic susceptibility locus shared between lumbar spine bone mineral density and birth weight.

Authors - Yu-Qian Song
View Article
mouseWB

Cell Mol Life Sci

Protease-independent control of parthanatos by HtrA2/Omi

Authors - Jonas Weiß
View Article