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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PDS5A Polyclonal antibody (17485-1-AP)

    WB analysis of HEK-293 using 17485-1-AP

    WB result of PDS5A antibody (17485-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDS5A transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using PDS5A Polyclonal antibody (17485-1-AP)

    WB analysis using 17485-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17485-1-AP (PDS5A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PDS5A Polyclonal antibody (17485-1-AP)

    WB analysis of HEK-293 using 17485-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17485-1-AP (PDS5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using PDS5A Polyclonal antibody (17485-1-AP)

    WB analysis of mouse kidney using 17485-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17485-1-AP (PDS5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using PDS5A Polyclonal antibody (17485-1-AP)

    WB analysis of COLO 320 using 17485-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17485-1-AP (PDS5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using PDS5A Polyclonal antibody (17485-1-AP)

    IHC staining of human colon using 17485-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17485-1-AP (PDS5A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using PDS5A Polyclonal antibody (17485-1-AP)

    IHC staining of human colon using 17485-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17485-1-AP (PDS5A Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using PDS5A Polyclonal antibody (17485-1-AP)

    IHC staining of human colon using 17485-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17485-1-AP (PDS5A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PDS5A Polyclonal antibody (17485-1-AP)

    IF Staining of A431 using 17485-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PDS5A antibody (17485-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

PDS5A Polyclonal antibody

PDS5A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17485-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SCC112, SCC 112, PIG54, PDS5, KIAA0648

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, mouse kidney tissue, COLO 320 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

17485-1-AP targets PDS5A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDS5A fusion protein Ag11525 相同性解析による交差性が予測される生物種
Full Name PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae)
Calculated molecular weight 1297 aa, 147 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC009650
Gene Symbol PDS5A
Gene ID (NCBI) 23244
RRIDAB_2162088
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ29RF7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PDS5A antibody 17485-1-APDownload protocol
IHC protocol for PDS5A antibody 17485-1-APDownload protocol
IF protocol for PDS5A antibody 17485-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

Requirement of Nek2a and cyclin A2 for Wapl-dependent removal of cohesin from prophase chromatin

Authors - Susanne Hellmuth
View Article
humanWB

EMBO Rep

Regulation of cohesin-mediated chromosome folding by PDS5 in mammals

Authors - Dingdang Yu
View Article