Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, mouse small intestine tissue, NIH/3T3 cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | human small intestine tissue, human colon tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 25 publications below |
| IHC | See 4 publications below |
Product Information
10026-1-AP targets PDK1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0025 Product name: Recombinant human PDK1 protein Source: e coli.-derived, Unknow Tag: N/A Domain: LF1(PDK-N) of BC039158 Sequence: 相同性解析による交差性が予測される生物種 |
| Full Name | pyruvate dehydrogenase kinase, isozyme 1 |
| Calculated molecular weight | 436 aa, 49 kDa |
| Observed molecular weight | 46-55 kDa |
| GenBank accession number | BC039158 |
| Gene Symbol | PDK1 |
| Gene ID (NCBI) | 5163 |
| RRID | AB_2161146 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15118 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PDK1(pyruvate dehydrogenase kinase isoform 1) is also named as PDHK1 and belongs to the PDK/BCKDK protein kinase family. It inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism (PMID:17683942). Starvation increases islet PDK activity (PMID:11723055). PDK1 has 2 isoforms with the MW of 49 and 52 kD, and a 28aa-transit-peptide in the N-terminal can be removed in the mature form. This antibody is speicfic to PDK1.
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Mitophagy mitigates mitochondrial fatty acid β-oxidation deficient cardiomyopathy. | ||
Cell Death Dis Mitochondrial dysfunction induces radioresistance in colorectal cancer by activating [Ca2+]m-PDP1-PDH-histone acetylation retrograde signaling. | ||
Cell Death Dis POU6F1 cooperates with RORA to suppress the proliferation of lung adenocarcinoma by downregulation HIF1A signaling pathway. | ||
Phytomedicine Modified Fuzheng Yiliu Decoction chemosensitizes colorectal cancer by inhibiting cancer stem cell metabolism and stemness through CAV1/ZNF460/GRP78 signaling. | ||
EBioMedicine Type Iγ phosphatidylinositol phosphate kinase promotes tumor growth by facilitating Warburg effect in colorectal cancer. | ||
Proc Natl Acad Sci U S A LncRNA IDH1-AS1 links the functions of c-Myc and HIF1α via IDH1 to regulate the Warburg effect. |