- Featured Product
- KD/KO Validated
PDHX Polyclonal antibody
PDHX Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10951-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | BxPC-3 cells, HEK-293 cells, SKOV-3 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PDHX Ready-To-Use IHC Kit. PDHX primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
10951-1-AP targets PDHX in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PDHX fusion protein Ag1389 相同性解析による交差性が予測される生物種 |
| Full Name | pyruvate dehydrogenase complex, component X |
| Calculated molecular weight | 54 kDa |
| Observed molecular weight | 54 kDa |
| GenBank accession number | BC010389 |
| Gene symbol | PDHX |
| Gene ID (NCBI) | 8050 |
| RRID | AB_2163102 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDHX antibody 10951-1-AP | Download protocol |
| IHC protocol for PDHX antibody 10951-1-AP | Download protocol |
| IP protocol for PDHX antibody 10951-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Sci Identification of PDHX as a metabolic target for esophageal squamous cell carcinoma.
| ||
Front Microbiol Singapore Grouper Iridovirus Induces Glucose Metabolism in Infected Cells by Activation of Mammalian Target of Rapamycin Signaling.
| ||
Int J Biol Sci Hypericin maintians PDX1 expression via the Erk pathway and protects islet β-cells against glucotoxicity and lipotoxicity. | ||
PLoS One Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3. | ||
Horm Metab Res Tendons from non-diabetic humans and rats harbor a population of insulin-producing, pancreatic beta cell-like cells. |
