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PDE7B Polyclonal antibody

PDE7B Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17771-1-AP

Synonyms

PDE7B, phosphodiesterase 7B



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

17771-1-AP targets PDE7B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDE7B fusion protein Ag12184 相同性解析による交差性が予測される生物種
Full Name phosphodiesterase 7B
Calculated molecular weight 450 aa, 52 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC075082
Gene symbol PDE7B
Gene ID (NCBI) 27115
RRIDAB_2268032
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDE7B(cAMP-specific 3',5'-cyclic phosphodiesterase 7B) belongs to the cyclic nucleotide phosphodiesterase family and PDE7 subfamily,which regulate intracellular levels of cAMP and cGMP, cyclic nucleotides that play important roles as second messengers in multiple physiologic processes. PDE7B gene is expressed predominantly in the brain and in a number of other tissues including liver, heart, thyroid glands, and skeletal muscle, but not expressed in blood leukocytes (PMID:12388353).

Protocols

Product Specific Protocols
WB protocol for PDE7B antibody 17771-1-APDownload protocol
IHC protocol for PDE7B antibody 17771-1-APDownload protocol
IF protocol for PDE7B antibody 17771-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IF

Neurobiol Aging

Silencing phosphodiesterase 7B gene by lentiviral-shRNA interference attenuates neurodegeneration and motor deficits in hemiparkinsonian mice.

Authors - Jose A Morales-Garcia
  • KD Validated
ratWB

Stem Cells

Phosphodiesterase7 Inhibition Activates Adult Neurogenesis in Hippocampus and Subventricular Zone in vitro and in vivo.

Authors - Jose A Morales-Garcia
ratWB

Stem Cells Transl Med

Phosphodiesterase 7 inhibition induces dopaminergic neurogenesis in hemiparkinsonian rats.

Authors - Jose A Morales-Garcia