Validation Data Gallery

Filter: AllWBIPIHCIF-P

WB analysis using 22063-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22063-1-AP (PDE6B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of mouse eye tissue using 22063-1-AP
WB results of PDE6B antibody (22063-1-AP) with WT mouse Eye and Prph2 (Rds) mutant mouse Eye (Negative control). Courtesy of Seongjin Seo, PhD, University of Iowa College of Medicine.
WB analysis of A549 using 22063-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 22063-1-AP (PDE6B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Y79 using 22063-1-AP
IP result of anti-PDE6B (IP:22063-1-AP, 4ug; Detection:22063-1-AP 1:1000) with Y79 cells lysate 2000ug.
IHC staining of rat eye using 22063-1-AP
Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 22063-1-AP (PDE6B antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat eye using 22063-1-AP
Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 22063-1-AP (PDE6B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse eye using 22063-1-AP
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 22063-1-AP (PDE6B antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse eye using 22063-1-AP
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 22063-1-AP (PDE6B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of rat eye using 22063-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using PDE6B antibody (22063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse eye using 22063-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PDE6B antibody (22063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse eye using 22063-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PDE6B antibody (22063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of rat eye using 22063-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using PDE6B antibody (22063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PDE6B Polyclonal antibody

PDE6B Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 22063-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

EC:3.1.4.35, GMP PDE beta, GMP-PDE beta, PDEB, Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse eye tissue, A549 cells, rat eye tissue
Positive IP detected in	Y79 cells
Positive IHC detected in	rat eye tissue, mouse eye tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	rat eye tissue, mouse eye tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 10 publications below
IHC	See 2 publications below
IF	See 6 publications below

Product Information

22063-1-AP targets PDE6B in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17139 Product name: Recombinant human PDE6B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-88 aa of BC000249 Sequence: MSLSEEQARSFLDQNPDFARQYFGKKLSPENVAAACEDGCPPDCDSLRDLCQVEESTALLELVQDMQESINMERVVFKVLRRLCTLLQ 相同性解析による交差性が予測される生物種
Full Name	phosphodiesterase 6B, cGMP-specific, rod, beta
Calculated molecular weight	854 aa, 98 kDa
Observed molecular weight	98 kDa
GenBank accession number	BC000249
Gene Symbol	PDE6B
Gene ID (NCBI)	5158
RRID	AB_2619668
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P35913
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PDE6 is a cGMP-specific PDE family and presents multicomponent enzyme complexes. The rod PDE6 enzyme is comprised of two catalytic subunits (PDE6A and PDE6B) and it is expressed in human lungs (PMID:20979602). PDE6B(Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta) is also named as PDEB and belongs to the cyclic nucleotide phosphodiesterase family. PDE6B also has a C-terminal CAAX motif for posttranslational processing involving lipidation, proteolysis, and carboxymethylation (PMID:8394243). Defects in PDE6B are the cause of retinitis pigmentosa type 40 (RP40)(PMID:22334370) and congenital stationary night blindness autosomal dominant type 2 (CSNBAD2) (PMID:8075643).

Protocols

Product Specific Protocols
IF protocol for PDE6B antibody 22063-1-AP	Download protocol
IHC protocol for PDE6B antibody 22063-1-AP	Download protocol
IP protocol for PDE6B antibody 22063-1-AP	Download protocol
WB protocol for PDE6B antibody 22063-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Sci Adv Synergistic antioxidant and gene supplementation for high-efficacy retinitis pigmentosa therapy. Authors - Fuhua Wu View Article
mouse	WB,IF	Research (Wash D C) Precise Correction of the Pde6b-L659P Mutation Causing Retinal Degeneration with Minimum Bystander Editing by Advanced Genome Editing Tools Authors - Zhiquan Liu View Article
mouse	WB,IF	Proc Natl Acad Sci U S A Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. Authors - Poppy Datta View Article
mouse	WB	NPJ Parkinsons Dis Multi-omic insight into the molecular mechanism of cuproptosis-related genes in the pathogenesis of Parkinson's disease. Authors - Ting Zhang View Article
human	WB	Stem Cell Res Ther Retinoic acid delays initial photoreceptor differentiation and results in a highly structured mature retinal organoid Authors - Carla Sanjurjo-Soriano View Article
		Zool Res Tubulin hyperglutamylation induces retinal degeneration by disrupting ciliary architecture and protein trafficking. Authors - Hao-Lin Wang View Article

Validation Data Gallery

WB analysis using 22063-1-AP

WB analysis of mouse eye tissue using 22063-1-AP

WB analysis of A549 using 22063-1-AP

IP experiment of Y79 using 22063-1-AP

IHC staining of rat eye using 22063-1-AP

IHC staining of rat eye using 22063-1-AP

IHC staining of mouse eye using 22063-1-AP

IHC staining of mouse eye using 22063-1-AP

IF Staining of rat eye using 22063-1-AP

IF Staining of mouse eye using 22063-1-AP

IF Staining of mouse eye using 22063-1-AP

IF Staining of rat eye using 22063-1-AP

PDE6B Polyclonal antibody

PDE6B Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Synergistic antioxidant and gene supplementation for high-efficacy retinitis pigmentosa therapy.

Precise Correction of the Pde6b-L659P Mutation Causing Retinal Degeneration with Minimum Bystander Editing by Advanced Genome Editing Tools

Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.

Multi-omic insight into the molecular mechanism of cuproptosis-related genes in the pathogenesis of Parkinson's disease.

Retinoic acid delays initial photoreceptor differentiation and results in a highly structured mature retinal organoid

Tubulin hyperglutamylation induces retinal degeneration by disrupting ciliary architecture and protein trafficking.

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