Validation Data Gallery

Filter: AllWBIHC

WB analysis of human brain using 16226-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 16226-1-AP (PDE4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A2780 using 16226-1-AP
WB result of PDE4A antibody (16226-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDE4A transfected A2780 cells.
WB analysis of human brain using 16226-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 16226-1-AP (PDE4A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 16226-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16226-1-AP (PDE4A antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 16226-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16226-1-AP (PDE4A antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

PDE4A Polyclonal antibody

PDE4A Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16226-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

3',5'-cyclic-AMP phosphodiesterase 4A, cAMP-specific phosphodiesterase 4A, DPDE2, EC:3.1.4.53, PDE4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human brain tissue, A2780 cells
Positive IHC detected in	human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 11 publications below
IHC	See 2 publications below

Product Information

16226-1-AP targets PDE4A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8018 Product name: Recombinant human PDE4A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 297-647 aa of BC019864 Sequence: MVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLRNMVHCADLSNPTKPLELYRQWTDRIMAEFFQQGDRERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVHPDAQEILDTLEDNRDWYYSAIRQSPSPPPEEESRGPGHPPLPDKFQFELTLEEEEEEEISMAQIPCTAQEALTAQGLSGVEEALDATIAWEASPAQESLEVMAQEASLEAELEAVYLTQQAQSTGSAPVAPDEFSSREEFVVAVSHSSPSALALQSPLLPAWRTLSVSEHAPGLPGLPSTAAEVEAQREHQAAKRACSACAGTFGEDTSALPAPGGGGSGGDPT 相同性解析による交差性が予測される生物種
Full Name	phosphodiesterase 4A, cAMP-specific (phosphodiesterase E2 dunce homolog, Drosophila)
Calculated molecular weight	98 kDa
Observed molecular weight	125-130 kDa
GenBank accession number	BC019864
Gene Symbol	PDE4A
Gene ID (NCBI)	5141
RRID	AB_2252090
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P27815
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for PDE4A antibody 16226-1-AP	Download protocol
WB protocol for PDE4A antibody 16226-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Acta Pharmacol Sin PDE4 inhibitor rolipram dynamically regulates the balance between D1-MSNs and D2-MSNs in the DMS to modulate abnormal "Go" behavior associated with alcohol addiction. Authors - Wei Zhao View Article
mouse	WB	Br J Pharmacol Involvement of 3',5'-cyclic inosine monophosphate in cystathionine γ-lyase-dependent regulation of the vascular tone. Authors - Emma Mitidieri View Article
mouse	WB	Biochem Pharmacol DC591017, a phosphodiesterase-4 (PDE4) inhibitor with robust anti-inflammation through regulating PKA-CREB signaling. Authors - Heng Li View Article
mouse	WB	Int Immunopharmacol Inhibition of phosphodiesterase-4 by rolipram alleviates anxiety-like behavior in mice with PTSD by modulating neuroinflammation and synaptic plasticity via cAMP signaling Authors - Yuanyuan Lin View Article
mouse	WB	Int J Mol Sci Phosphodiesterases Expression during Murine Cardiac Development. Authors - Thays Maria da Conceição Silva Carvalho View Article
mouse	WB	Int J Neuropsychopharmacol Upregulation of Phosphodiesterase 7A Contributes to Concurrent Pain and Depression via Inhibition of cAMP-PKA-CREB-BDNF Signaling and Neuroinflammation in the Hippocampus of Mice Authors - Shi-Cai Chen View Article

製品

アプリケーション

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サポート

カスタム対応

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企業情報

抗体

免疫アッセイ

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Validation Data Gallery

WB analysis of human brain using 16226-1-AP

WB analysis of A2780 using 16226-1-AP

WB analysis of human brain using 16226-1-AP

IHC staining of human placenta using 16226-1-AP

IHC staining of human placenta using 16226-1-AP

PDE4A Polyclonal antibody

PDE4A Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

PDE4 inhibitor rolipram dynamically regulates the balance between D1-MSNs and D2-MSNs in the DMS to modulate abnormal "Go" behavior associated with alcohol addiction.

Involvement of 3',5'-cyclic inosine monophosphate in cystathionine γ-lyase-dependent regulation of the vascular tone.

DC591017, a phosphodiesterase-4 (PDE4) inhibitor with robust anti-inflammation through regulating PKA-CREB signaling.

Inhibition of phosphodiesterase-4 by rolipram alleviates anxiety-like behavior in mice with PTSD by modulating neuroinflammation and synaptic plasticity via cAMP signaling

Phosphodiesterases Expression during Murine Cardiac Development.

Upregulation of Phosphodiesterase 7A Contributes to Concurrent Pain and Depression via Inhibition of cAMP-PKA-CREB-BDNF Signaling and Neuroinflammation in the Hippocampus of Mice

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Validation Data Gallery

WB analysis of human brain using 16226-1-AP

WB analysis of A2780 using 16226-1-AP

WB analysis of human brain using 16226-1-AP

IHC staining of human placenta using 16226-1-AP

IHC staining of human placenta using 16226-1-AP

PDE4A Polyclonal antibody

PDE4A Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

PDE4 inhibitor rolipram dynamically regulates the balance between D1-MSNs and D2-MSNs in the DMS to modulate abnormal "Go" behavior associated with alcohol addiction.

Involvement of 3',5'-cyclic inosine monophosphate in cystathionine γ-lyase-dependent regulation of the vascular tone.

DC591017, a phosphodiesterase-4 (PDE4) inhibitor with robust anti-inflammation through regulating PKA-CREB signaling.

Inhibition of phosphodiesterase-4 by rolipram alleviates anxiety-like behavior in mice with PTSD by modulating neuroinflammation and synaptic plasticity via cAMP signaling

Phosphodiesterases Expression during Murine Cardiac Development.

Upregulation of Phosphodiesterase 7A Contributes to Concurrent Pain and Depression via Inhibition of cAMP-PKA-CREB-BDNF Signaling and Neuroinflammation in the Hippocampus of Mice

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