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- KD/KO Validated
PDE2A Polyclonal antibody
PDE2A Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 55306-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | mouse brain tissue, mouse heart tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
55306-1-AP targets PDE2A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | phosphodiesterase 2A, cGMP-stimulated |
| Calculated molecular weight | 106 kDa |
| Observed molecular weight | 100-106 kDa |
| GenBank accession number | NM_002599 |
| Gene symbol | PDE2A |
| Gene ID (NCBI) | 5138 |
| RRID | AB_11182279 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PDE2A, also named as cGSPDE and CGS-PDE, belongs to the cyclic nucleotide phosphodiesterase family and PDE2 subfamily. It is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. This antibody is specific to PDE2A.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDE2A antibody 55306-1-AP | Download protocol |
| IHC protocol for PDE2A antibody 55306-1-AP | Download protocol |
| IP protocol for PDE2A antibody 55306-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv RasGRP1 is a causal factor in the development of l-DOPA-induced dyskinesia in Parkinson's disease. | ||
Br J Pharmacol 17β-estradiol reduces mitochondrial cAMP content and cytochrome oxidase activity in a phosphodiesterase 2-dependent manner. | ||
Biochem Pharmacol Hydrogen sulfide-mediated stimulation of mitochondrial electron transport involves inhibition of the mitochondrial phosphodiesterase 2A, elevation of cAMP and activation of protein kinase A. | ||
Front Pharmacol Proteomics Study Reveals the Anti-Depressive Mechanisms and the Compatibility Advantage of Chaihu-Shugan-San in a Rat Model of Chronic Unpredictable Mild Stress. | ||
Commun Biol Phosphodiesterase 2A2 regulates mitochondria clearance through Parkin-dependent mitophagy.
| ||
JCI Insight Phosphodiesterase 2A as a therapeutic target to restore cardiac neurotransmission during sympathetic hyperactivity. |
