Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, H7ES cells, mouse liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 4 publications below |
Product Information
10725-1-AP targets PDCD2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, zebrafish samples.
| Tested Reactivity | human, mouse, zebrafish |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1139 Product name: Recombinant human PDCD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-344 aa of BC008378 Sequence: MAAAGARPVELGFAESAPAWRLRSEQFPSKVGGRPAWLGAAGLPGPQALACELCGRPLSFLLQVYAPLPGRPDAFHRCIFLFCCREQPCCAGLRVFRNQLPRKNDFYSYEPPSENPPPETGESVCLQLKSGAHLCRVCGCLGPKTCSRCHKAYYCSKEHQTLDWRLGHKQACAQPDHLDHIIPDHNFLFPEFEIVIETEDEIMPEVVEKEDYSEIIGSMGEALEEELDSMAKHESREDKIFQKFKTQIALEPEQILRYGRGIAPIWISGENIPQEKDIPDCPCGAKRILEFQVMPQLLNYLKADRLGKSIDWGILAVFTCAESCSLGTGYTEEFVWKQDVTDTP 相同性解析による交差性が予測される生物種 |
| Full Name | programmed cell death 2 |
| Calculated molecular weight | 39 kDa |
| Observed molecular weight | 37-39 kDa, 45 kDa |
| GenBank accession number | BC008378 |
| Gene Symbol | PDCD2 |
| Gene ID (NCBI) | 5134 |
| RRID | AB_2236895 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16342 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Programmed Cell Death 2 (PDCD2) is widely expressed in embryonic and adult tissues but with undefined function yet. Recent observation uncovered its role in hematopoietic stem cell differentiation during development. Catalog# 10725-1-AP is a rabbit polyclonal antibody raised against full-length PDCD2 of human origin.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PDCD2 antibody 10725-1-AP | Download protocol |
| IP protocol for PDCD2 antibody 10725-1-AP | Download protocol |
| WB protocol for PDCD2 antibody 10725-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics Quantitative proteomic analysis of cellular protein modulation upon inhibition of the NEDD8-activating enzyme by MLN4924. | ||
Br J Haematol MicroRNA 200a inhibits erythroid differentiation by targeting PDCD4 and THRB. | ||
Sci Rep Single-cell RNA-seq reveals distinct injury responses in different types of DRG sensory neurons. | ||
Stem Cells Dev PDCD2 controls hematopoietic stem cell differentiation during development. | ||
Dev Biol PDCD2 is essential for inner cell mass development and embryonic stem cell maintenance.
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