Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, mouse heart tissue, HepG2 cells, RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IHC | See 17 publications below |
Product Information
18251-1-AP targets PD-L2/CD273 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13039 Product name: Recombinant human PDCD1LG2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-273 aa of BC074766 Sequence: MIFLLLMLSLELQLHQIAALFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRELTLASIDLQSQMEPRTHPTWLLHIFIPSCIIAFIFIATVIALRKQLCQKLYSSKDTTKRPVTTTKREVNSAI 相同性解析による交差性が予測される生物種 |
| Full Name | programmed cell death 1 ligand 2 |
| Calculated molecular weight | 273 aa, 31 kDa |
| Observed molecular weight | 40-50 kDa, 28 kDa |
| GenBank accession number | BC074766 |
| Gene Symbol | PD-L2 |
| Gene ID (NCBI) | 80380 |
| RRID | AB_2878523 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BQ51 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Programmed cell death ligand 2 (PD-L2, also known as CD273, or B7-DC), is one of the B7 family molecules which are type I transmembrane proteins belonging to the immunoglobulin superfamily. PD-L2 was identified both by its homology to PD-L1 and by analysis of genes differentially expressed in libraries generated from dendritic cells (DC) and activated macrophages (PMID: 14515254). PD-L2 is a second ligand for PD-1, a costimulatory molecule that plays an inhibitory role in regulating T cell activation in the periphery.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PD-L2/CD273 antibody 18251-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B PKCα inhibitors promote breast cancer immune evasion by maintaining PD-L1 stability | ||
Cell Death Discov Cisplatin remodels the tumor immune microenvironment via the transcription factor EB in ovarian cancer. | ||
J Transl Med Treatment with eFT-508 increases chemosensitivity in breast cancer cells by modulating the tumor microenvironment. | ||
Cell Rep The hexosamine biosynthetic pathway drives tumor immune evasion via translational control of PD-L1 at the elongation level | ||
Aging Cell Targeting amphiregulin (AREG) derived from senescent stromal cells diminishes cancer resistance and averts programmed cell death 1 ligand (PD-L1)-mediated immunosuppression. |