• Featured Product
  • KD/KO Validated

PALMD Polyclonal antibody

PALMD Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16531-1-AP

Synonyms

C1orf11, PALMD, palmdelphin, PALML, Paralemnin like protein



Tested Applications

Positive WB detected inHeLa cells, mouse lung tissue
Positive IP detected inmouse heart tissue,
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PALMD Ready-To-Use IHC Kit. PALMD primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16531-1-AP targets PALMD in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PALMD fusion protein Ag9796 相同性解析による交差性が予測される生物種
Full Name palmdelphin
Calculated molecular weight 551 aa, 63 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC012570
Gene symbol PALMD
Gene ID (NCBI) 54873
RRIDAB_2236613
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Palmdelphin, encoded by PALMD gene, is a newly identified cytosolic isoform of paralemmin-1, a lipid raft-associated protein implicated in cell shape control. Paralemmin (Palm) is a prenyl-palmitoyl anchored membrane protein that can drive membrane and process formation in neurons. Previous finding suggest that palmdelphin may peripherally associate with endomembranes or cytoskeleton-linked structures. Palmdelphin appears to be 80 kDa in Western blotting, larger than the predicated of 63 kDa, and this may be due to the acidic nature of this protein or its posttranslational modification.

Protocols

Product Specific Protocols
WB protocol for PALMD antibody 16531-1-APDownload protocol
IHC protocol for PALMD antibody 16531-1-APDownload protocol
IP protocol for PALMD antibody 16531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Hum Mol Genet

Interactome network analysis identifies multiple caspase-6 interactors involved in the pathogenesis of HD.

Authors - Sean-Patrick Riechers
humanWB

Cell Death Dis

Palmdelphin, a novel target of p53 with Ser46 phosphorylation, controls cell death in response to DNA damage.

Authors - N Dashzeveg
  • KD Validated
humanWB

Front Oncol

Low Level of PALMD Contributes to the Metastasis of Uveal Melanoma.

Authors - Min-Yun Cai
  • KD Validated
humanWB,IF

bioRxiv

Role of a Pdlim5:PalmD complex in directing dendrite morphology

Authors - Yogesh Srivastava
mouseIF,IHC

Cell Rep

Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease

Authors - Min Yu