CoraLite®594-conjugated PAI-1 Monoclonal antibody
PAI-1 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
human, pig
Applications
IF
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1H4A5
Cat no : CL594-66261
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy SERPINE1 Ready-To-Use IHC Kit. SERPINE1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66261-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL594-66261 targets PAI-1 in IF applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PAI-1 fusion protein Ag22411 相同性解析による交差性が予測される生物種 |
| Full Name | serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 |
| Calculated molecular weight | 402 aa, 45 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC010860 |
| Gene symbol | SERPINE1 |
| Gene ID (NCBI) | 5054 |
| RRID | AB_2883545 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tissue plasminogen activator (tPA) and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 PAI-1 antibody CL594-66261 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
