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CoraLite® Plus 488-conjugated PADI4 Polyclonal antibody
PADI4 Polyclonal Antibody for IF, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-17373
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | A549 cells |
| Positive FC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-17373 targets PADI4 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PADI4 fusion protein Ag11068 相同性解析による交差性が予測される生物種 |
| Full Name | peptidyl arginine deiminase, type IV |
| Calculated molecular weight | 663 aa, 74 kDa |
| Observed molecular weight | 67 kDa |
| GenBank accession number | BC025718 |
| Gene symbol | PADI4 |
| Gene ID (NCBI) | 23569 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PADI4, also named as PADI5 and PDI5, catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. PADI4 is expressed in hematologic and rheumatoid arthritis synovial tissues. PADI4 haplotype associated with susceptibility to rheumatoid arthritis increases production of citrullinated peptides, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Immunohistochemistry detected significant PADI4 expression in various malignancies and PADI4 expression was not detected in non-tumor tissues.(PMID: 19183436)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PADI4 antibody CL488-17373 | Download protocol |
| FC protocol for CL Plus 488 PADI4 antibody CL488-17373 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
