Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 10639-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 10639-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 10639-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 10639-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 10639-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 10639-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 10639-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10639-1-AP (PACSIN3 antibody) at dilution of 1:100 (under 25x lens).
IHC staining of human heart using 10639-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10639-1-AP (PACSIN3 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 10639-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10639-1-AP (PACSIN3 Antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HeLa using 10639-1-AP
Immunofluorescent analysis of HeLa cells using 10639-1-AP (PACSIN3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PACSIN3 Polyclonal antibody

PACSIN3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10639-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SH3 domain-containing protein 6511, Protein kinase C and casein kinase substrate in neurons protein 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse heart tissue, mouse liver tissue, mouse lung tissue, mouse skeletal muscle tissue, rat brain tissue
Positive IHC detected in	human breast cancer tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

10639-1-AP targets PACSIN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1006 Product name: Recombinant human PACSIN3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC007914 Sequence: MAPEEDAGGEALGGSFWEAGNYRRTVQRVEDGHRLCGDLVSCFQERARIEKAYAQQLADWARKWRGTVEKGPQYGTLEKAWHAFFTAAERLSALHLEVREKLQGQDSERVRAWQRGAFHRPVLGGFRESRAAEDGFRKAQKPWLKRLKEVEASKKSYHAARKDEKTAQTRESHAKADSAVSQEQLRKLQERVERCAKEAEKTKAQYEQTLAELHRYTPRYMEDMEQAFETCQAAERQRLLFFKDMLLTLHQHLDLSSSEKFHELHRDLHQGIEAASDEEDLRWWRSTHGPGMAMNWPQFE 相同性解析による交差性が予測される生物種
Full Name	protein kinase C and casein kinase substrate in neurons 3
Calculated molecular weight	48 kDa
Observed molecular weight	48 kDa
GenBank accession number	BC007914
Gene Symbol	PACSIN3
Gene ID (NCBI)	29763
RRID	AB_2159453
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UKS6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
FC protocol for PACSIN3 antibody 10639-1-AP	Download protocol
IF protocol for PACSIN3 antibody 10639-1-AP	Download protocol
IHC protocol for PACSIN3 antibody 10639-1-AP	Download protocol
WB protocol for PACSIN3 antibody 10639-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Brain Biallelic truncating variants in PACSIN3 cause childhood-onset myopathy with hyperCKaemia Authors - Felix Distelmaier View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 10639-1-AP

WB analysis of rat brain using 10639-1-AP

WB analysis of mouse heart using 10639-1-AP

WB analysis of mouse skeletal muscle using 10639-1-AP

WB analysis of mouse liver using 10639-1-AP

WB analysis of mouse lung using 10639-1-AP

IHC staining of human breast cancer using 10639-1-AP

IHC staining of human heart using 10639-1-AP