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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

PACS2 Polyclonal antibody

PACS2 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 30172-1-AP

Synonyms

FLJ25488, KIAA0602, PACS 2, PACS1 like protein, PACS1L, PACS2

Validation Data Gallery

Applications:
  • WB analysis of HCT 116 using 30172-1-AP

    WB analysis of HCT 116 using 30172-1-AP

    WB result of PACS2 antibody (30172-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACS2 transfected HCT 116 cells.

  • WB analysis using 30172-1-AP

    WB analysis using 30172-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30172-1-AP (PACS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HCT 116 using 30172-1-AP

    IP experiment of HCT 116 using 30172-1-AP

    IP result of anti-PACS2 (IP:30172-1-AP, 4ug; Detection:30172-1-AP 1:1500) with HCT 116 cells lysate 2440 ug.

  • IF Staining of HCT 116 using 30172-1-AP

    IF Staining of HCT 116 using 30172-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using PACS2 antibody (30172-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHCT 116 cells, HuH-7 cells
Positive IP detected inHCT 116 cells
Positive IF detected inHCT 116 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

30172-1-AP targets PACS2 in WB, IP, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PACS2 fusion protein Ag32902 相同性解析による交差性が予測される生物種
Full Name phosphofurin acidic cluster sorting protein 2
Calculated molecular weight98kd
Observed molecular weight100-130 kDa
GenBank accession numberNM_001100913
Gene symbol PACS2
Gene ID (NCBI) 23241
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PACS2, also named PACS1L or KIAA0602, is one of PACS (phosphofurin acidic cluster sorting) proteins which are one such family of multifunctional membrane traffic regulators that mediate organ homoeostasis and have important roles in diverse pathologies and disease states. PACS2 is a multifunctional sorting protein that controls the endoplasmic reticulum (ER)-mitochondria communication, ER homeostasis, and apoptosis. PACS2 may be involved in ion channel trafficking, directing acidic cluster-containing ion channels to distinct subcellular compartments.

Protocols

Product Specific Protocols
WB protocol for PACS2 antibody 30172-1-APDownload protocol
IF protocol for PACS2 antibody 30172-1-APDownload protocol
IP protocol for PACS2 antibody 30172-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols