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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated PA2G4 Monoclonal antibody

PA2G4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3G10D9

Cat no : CL594-66055

Synonyms

EBP1, ErbB3 binding protein 1, HG4 1, p38 2G4, PA2G4

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL594-66055

    IF Staining of HeLa using CL594-66055

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66055 (PA2G4 antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PA2G4 Ready-To-Use IHC Kit. PA2G4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66055-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-66055 is PA2G4 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PA2G4 fusion protein Ag7850 相同性解析による交差性が予測される生物種
Full Name proliferation-associated 2G4, 38kDa
Calculated molecular weight 44 kDa
Observed molecular weight 38-42 kDa, 48 kDa
GenBank accession numberBC001951
Gene symbol PA2G4
Gene ID (NCBI) 5036
RRIDAB_2883494
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a mouse mono-clonal antibody raised against full length PA2G4 of human origin.

Protocols

Product Specific Protocols
IF protocol for CL594 PA2G4 antibody CL594-66055Download protocol
Standard Protocols
Click here to view our Standard Protocols