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  • Featured Product
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PA2G4 Monoclonal antibody

PA2G4 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G10D9

Cat no : 66055-1-Ig

Synonyms

EBP1, ErbB3 binding protein 1, HG4 1, p38 2G4, PA2G4

Validation Data Gallery

Applications:
  • WB analysis using 66055-1-Ig

    WB analysis using 66055-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66055-1-Ig (PA2G4 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HEK-293 using 66055-1-Ig

    WB analysis of HEK-293 using 66055-1-Ig

    WB result of PA2G4 antibody (66055-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PA2G4 transfected HEK-293 cells.

  • IHC staining of human breast cancer using 66055-1-Ig

    IHC staining of human breast cancer using 66055-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66055-1-Ig (PA2G4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PA2G4 antibody included
  • IHC staining of human breast cancer using 66055-1-Ig

    IHC staining of human breast cancer using 66055-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66055-1-Ig (PA2G4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PA2G4 antibody included
  • IF Staining of HeLa using 66055-1-Ig

    IF Staining of HeLa using 66055-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PA2G4 antibody (66055-1-Ig, Clone: 3G10D9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells, HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PA2G4 Ready-To-Use IHC Kit. PA2G4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66055-1-Ig targets PA2G4 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PA2G4 fusion protein Ag7850 相同性解析による交差性が予測される生物種
Full Name proliferation-associated 2G4, 38kDa
Calculated molecular weight 44 kDa
Observed molecular weight 38-42 kDa, 48 kDa
GenBank accession numberBC001951
Gene symbol PA2G4
Gene ID (NCBI) 5036
RRIDAB_11042597
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a mouse mono-clonal antibody raised against full length PA2G4 of human origin.

Protocols

Product Specific Protocols
WB protocol for PA2G4 antibody 66055-1-IgDownload protocol
IHC protocol for PA2G4 antibody 66055-1-IgDownload protocol
IF protocol for PA2G4 antibody 66055-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Stem Cell

HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis.

Authors - Kaosheng Lv
View Article