Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
11300-1-AP targets P2RY6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | P2RY6 fusion protein Ag1820 相同性解析による交差性が予測される生物種 |
| Full Name | pyrimidinergic receptor P2Y, G-protein coupled, 6 |
| Calculated molecular weight | 36 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC009391 |
| Gene Symbol | P2RY6 |
| Gene ID (NCBI) | 5031 |
| RRID | AB_2283156 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15077 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs). P2RY6 is responsive to UDP, partially responsive to UTP and ADP, and not responsive to ATP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. (PMID: 8670200; 21724990)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for P2RY6 antibody 11300-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Radiat Res Involvement of P2Y6 Receptor in p38 MAPK-Mediated COX-2 Expression in Response to UVB Irradiation of Human Keratinocytes. | ||
Radiat Res Involvement of P2Y6 receptor in p38 MAPK-mediated COX-2 expression in response to UVB irradiation of human keratinocytes. |