Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, L02 cells |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | mouse placenta tissue, human breast cancer tissue, human hysteromyoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 12 publications below |
| IHC | See 11 publications below |
| IF | See 8 publications below |
| IP | See 1 publications below |
Product Information
23045-1-AP targets OXTR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19074 Product name: Recombinant human OXTR protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 327-389 aa of BC137443 Sequence: WIYMLFTGHLFHELVQRFLCCSASYLKGRRLGETSASKKSNSSSFVLSHRSSSQRSCSQPSTA 相同性解析による交差性が予測される生物種 |
| Full Name | OXTR |
| Calculated molecular weight | 389 aa, 43 kDa |
| Observed molecular weight | 46 kDa, 67 kDa |
| GenBank accession number | BC137443 |
| Gene Symbol | OXTR |
| Gene ID (NCBI) | 5021 |
| RRID | AB_2827425 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30559 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Oxytocin (OXT) is a neurohypophysial hormone that plays a role in lactation and parturition and in the central nervous system as a neurotransmitter involved in sex and maternal behavior (PMID: 1852313; 10811917). OXT acts through its receptor, OXTR, which belongs to the G protein-coupled 7-transmembrane receptor family. OXTR is expressed in the endometrium, myometrium, and mammary gland (PMID: 1313946). This antibody is raised against the C-terminal region of human OXTR. Two immunoreactive OXTR bands were found in the fat tissue, an unglycosylated 43 kDa form and a mature glycosylated 67 kDa form (PMID:32819381).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OXTR antibody 23045-1-AP | Download protocol |
| IHC protocol for OXTR antibody 23045-1-AP | Download protocol |
| IF protocol for OXTR antibody 23045-1-AP | Download protocol |
| IP protocol for OXTR antibody 23045-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Physiol (Oxf) Oxytocin modulates neural processing of mitral/tufted cells in the olfactory bulb. | ||
Antioxidants (Basel) ΔMST and the Regulation of Cardiac CSE and OTR Expression in Trauma and Hemorrhage. | ||
Biochem Pharmacol Synergistic antitumor activity of 5-fluorouracil and atosiban against microsatellite stable colorectal cancer through restoring GATA3.
| ||
Front Pharmacol The Role of Formyl Peptide Receptor 1 in Uterine Contraction During Parturition. |