Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, HuH-7 cells, mouse brain tissue, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| IF | See 4 publications below |
| ChIP | See 1 publications below |
Product Information
14751-1-AP targets OSBPL2 in WB, IHC, IF-P, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | OSBPL2 fusion protein Ag6496 相同性解析による交差性が予測される生物種 |
| Full Name | oxysterol binding protein-like 2 |
| Calculated molecular weight | 55 kDa |
| Observed molecular weight | 51 kDa, 56 kDa |
| GenBank accession number | BC000296 |
| Gene Symbol | OSBPL2 |
| Gene ID (NCBI) | 9885 |
| RRID | AB_2156092 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H1P3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OSBPL2 antibody 14751-1-AP | Download protocol |
| IHC protocol for OSBPL2 antibody 14751-1-AP | Download protocol |
| IF protocol for OSBPL2 antibody 14751-1-AP | Download protocol |
| IP protocol for OSBPL2 antibody 14751-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy OSBPL2 mutations impair autophagy and lead to hearing loss, potentially remedied by rapamycin. | ||
JCI Insight Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via Sonic Hedgehog signaling. | ||
Genet Med Identification of OSBPL2 as a novel candidate gene for progressive nonsyndromic hearing loss by whole-exome sequencing. | ||
Mol Cell Endocrinol Oxysterol-related-binding-protein related Protein-2 (ORP2) regulates cortisol biosynthesis and cholesterol homeostasis.
| ||
Exp Cell Res OSBPL2 deficiency upregulate SQLE expression increasing intracellular cholesterol and cholesteryl ester by AMPK/SP1 and SREBF2 signalling pathway.
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