Validation Data Gallery

Filter: AllWBIPIHCIF-P

WB analysis using 14751-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 14751-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14751-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HuH-7 using 14751-1-AP
HuH-7 cells were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14751-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14751-1-AP
IP result of anti-OSBPL2 (IP:14751-1-AP, 4ug; Detection:14751-1-AP 1:500) with HeLa cells lysate 880ug.
IHC staining of human ovary cancer using 14751-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 14751-1-AP (OSBPL2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 14751-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using OSBPL2 antibody (14751-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

OSBPL2 Polyclonal antibody

OSBPL2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 14751-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, IP, ChIP, ELISA

Synonyms

OSBP-related protein 2, OSBP related protein 2, ORP-2, ORP2, ORP 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (11)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, A549 cells, HuH-7 cells, mouse brain tissue, HepG2 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human ovary cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 7 publications below
IF	See 5 publications below
ChIP	See 1 publications below

Product Information

14751-1-AP targets OSBPL2 in WB, IHC, IF-P, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6496 Product name: Recombinant human OSBPL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 128-480 aa of BC000296 Sequence: ERMQSVAAFAVSAVASQWERTGKPFNPLLGETYELIREDLGFRFISEQVSHHPPISAFHSEGLNHDFLFHGSIYPKLKFWGKSVEAEPRGTITLELLKHNEAYTWTNPTCCVHNVIIGKLWIEQYGTVEILNHRTGHKCVLHFKPCGLFGKELHKVEGHIQDKNKKKLFMIYGKWTECLWGIDPVSYESFKKQERRGDHLRKAKLDEDSGKADSDVADDVPVAQETVQVIPGSKLLWRINTRPPNSAQMYNFTSFTVSLNELETGMEKTLPPTDCRLRPDIRGMENGNMDLASQEKERLEEKQREARRERAKEEAEWQTRWFYPGNNPYTGTPDWLYAGDYFERNFSDCPDIY 相同性解析による交差性が予測される生物種
Full Name	oxysterol binding protein-like 2
Calculated molecular weight	55 kDa
Observed molecular weight	51 kDa, 56 kDa
GenBank accession number	BC000296
Gene Symbol	OSBPL2
Gene ID (NCBI)	9885
RRID	AB_2156092
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H1P3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for OSBPL2 antibody 14751-1-AP	Download protocol
IHC protocol for OSBPL2 antibody 14751-1-AP	Download protocol
IP protocol for OSBPL2 antibody 14751-1-AP	Download protocol
WB protocol for OSBPL2 antibody 14751-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Protein Cell ORP8 acts as a lipophagy receptor to mediate lipid droplet turnover Authors - Maomao Pu View Article
human	WB,IF	Autophagy OSBPL2 mutations impair autophagy and lead to hearing loss, potentially remedied by rapamycin. Authors - Young Ik Koh View Article
mouse	IF	JCI Insight Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via Sonic Hedgehog signaling. Authors - Hairong Shi View Article
	WB	Genet Med Identification of OSBPL2 as a novel candidate gene for progressive nonsyndromic hearing loss by whole-exome sequencing. Authors - Guangqian Xing View Article
human	IF,ChIP	Mol Cell Endocrinol Oxysterol-related-binding-protein related Protein-2 (ORP2) regulates cortisol biosynthesis and cholesterol homeostasis. Authors - Tamara Escajadillo KD Validated View Article
human	WB	Exp Cell Res OSBPL2 deficiency upregulate SQLE expression increasing intracellular cholesterol and cholesteryl ester by AMPK/SP1 and SREBF2 signalling pathway. Authors - Cui Zhang KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14751-1-AP

WB analysis of A549 using 14751-1-AP

WB analysis of HeLa using 14751-1-AP

WB analysis of HuH-7 using 14751-1-AP

WB analysis of mouse brain using 14751-1-AP

IP experiment of HeLa using 14751-1-AP

IHC staining of human ovary cancer using 14751-1-AP

IF Staining of mouse brain using 14751-1-AP

OSBPL2 Polyclonal antibody

OSBPL2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

ORP8 acts as a lipophagy receptor to mediate lipid droplet turnover

OSBPL2 mutations impair autophagy and lead to hearing loss, potentially remedied by rapamycin.

Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via Sonic Hedgehog signaling.

Identification of OSBPL2 as a novel candidate gene for progressive nonsyndromic hearing loss by whole-exome sequencing.

Oxysterol-related-binding-protein related Protein-2 (ORP2) regulates cortisol biosynthesis and cholesterol homeostasis.

OSBPL2 deficiency upregulate SQLE expression increasing intracellular cholesterol and cholesteryl ester by AMPK/SP1 and SREBF2 signalling pathway.