Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 11096-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11096-1-AP (OSBP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 11096-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 11096-1-AP (OSBP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 11096-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11096-1-AP (OSBP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa cells using 11096-1-AP
IP result of anti-OSBP (11096-1-AP for IP and Detection).
IHC staining of human ovary tumor using 11096-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11096-1-AP (OSBP antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HeLa using 11096-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11096-1-AP (OSBP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

OSBP Polyclonal antibody

OSBP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11096-1-AP

Publications(23)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Oxysterol-binding protein 1, Oxysterol binding protein 1, oxysterol binding protein, OSBP1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (23)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, rat kidney tissue, mouse kidney tissue, HeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 20 publications below
IF	See 9 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

11096-1-AP targets OSBP in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rabbit, yeast
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1578 Product name: Recombinant human OSBP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 508-807 aa of BC011581 Sequence: LEENGYRSLCEQVSHHPPAAAHHAESKNGWTLRQEIKITSKFRGKYLSIMPLGTIHCIFHATGHHYTWKKVTTTVHNIIVGKLWIDQSGEIDIVNHKTGDKCNLKFVPYSYFSRDVARKVTGEVTDPSGKVHFALLGTWDEKMECFKVQPVIGENGGDARQRGHEAEESRVMLWKRNPLPKNAENMYYFSELALTLNAWESGTAPTDSRLRPDQRLMENGRWDEANAEKQRLEEKQRLSRKKREAEAMKATEDGTPYDPYKALWFERKKDPVTKELTHIYRGEYWECKEKQDWSSCPDIF 相同性解析による交差性が予測される生物種
Full Name	oxysterol binding protein
Calculated molecular weight	89 kDa
Observed molecular weight	89 kDa
GenBank accession number	BC011581
Gene Symbol	OSBP
Gene ID (NCBI)	5007
RRID	AB_2158220
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P22059
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for OSBP antibody 11096-1-AP	Download protocol
IHC protocol for OSBP antibody 11096-1-AP	Download protocol
IF protocol for OSBP antibody 11096-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Science LYVAC/PDZD8 is a lysosomal vacuolator Authors - Haoxiang Yang View Article
human	WB,IF	Nature A phosphoinositide signalling pathway mediates rapid lysosomal repair Authors - Jay Xiaojun Tan View Article
human	WB,IF	Gastroenterology Oxysterol-Binding Protein Is a Phosphatidylinositol 4-Kinase Effector Required for HCV Replication Membrane Integrity and Cholesterol Trafficking. Authors - Hongliang Wang View Article
human	WB	Cell Host Microbe The antiviral effector IFITM3 disrupts intracellular cholesterol homeostasis to block viral entry. Authors - Samad Amini-Bavil-Olyaee View Article
mouse	WB	Mol Metab Multi-Omics after O-GlcNAc Alteration Identified Cellular Processes Promoting Aneuploidy after Loss of O-GlcNAc Transferase Authors - Samuel S Boyd View Article
human	WB,IF	Phytomedicine LW-213, a derivative of wogonin, triggers reticulophagy-mediated cell death in NSCLC via lysosomal damage combined with NPC1 inhibition Authors - Hongzheng Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11096-1-AP

WB analysis of rat kidney using 11096-1-AP

WB analysis of mouse kidney using 11096-1-AP

IP experiment of HeLa cells using 11096-1-AP

IHC staining of human ovary tumor using 11096-1-AP

IF Staining of HeLa using 11096-1-AP

OSBP Polyclonal antibody

OSBP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

LYVAC/PDZD8 is a lysosomal vacuolator

A phosphoinositide signalling pathway mediates rapid lysosomal repair

Oxysterol-Binding Protein Is a Phosphatidylinositol 4-Kinase Effector Required for HCV Replication Membrane Integrity and Cholesterol Trafficking.

The antiviral effector IFITM3 disrupts intracellular cholesterol homeostasis to block viral entry.

Multi-Omics after O-GlcNAc Alteration Identified Cellular Processes Promoting Aneuploidy after Loss of O-GlcNAc Transferase

LW-213, a derivative of wogonin, triggers reticulophagy-mediated cell death in NSCLC via lysosomal damage combined with NPC1 inhibition