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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ONECUT1 Polyclonal antibody

ONECUT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25137-1-AP

Synonyms

Hepatocyte nuclear factor 6, HNF 6, HNF6, HNF6A, One cut domain family member 1, one cut homeobox 1, ONECUT1

Validation Data Gallery

Applications:
  • WB analysis of mouse liver using 25137-1-AP

    WB analysis of mouse liver using 25137-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25137-1-AP (ONECUT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 25137-1-AP

    IHC staining of human liver cancer using 25137-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human liver cancer using 25137-1-AP

    IHC staining of human liver cancer using 25137-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human liver using 25137-1-AP

    IHC staining of human liver using 25137-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 25137-1-AP

    IHC staining of human liver using 25137-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human liver cancer using 25137-1-AP

    IHC staining of human liver cancer using 25137-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver cancer using 25137-1-AP

    IHC staining of human liver cancer using 25137-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human liver cancer using 25137-1-AP

    IHC staining of human liver cancer using 25137-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 25137-1-AP

    IHC staining of human liver cancer using 25137-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 25137-1-AP is ONECUT1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ONECUT1 fusion protein Ag18250 相同性解析による交差性が予測される生物種
Full Name one cut homeobox 1
Calculated molecular weight 465 aa, 51 kDa
Observed molecular weight 51-55 kDa
GenBank accession numberBC140830
Gene symbol ONECUT1
Gene ID (NCBI) 3175
RRIDAB_2879918
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ONECUT1, also named as Hepatocyte nuclear factor 6 (HNF6), is a 465 amino acid protein, which contains one CUT DNA-binding domain and one homeobox DNA-binding domain. ONECUT1 localizes in the nucleus and belongs to the CUT homeobox family. ONECUT1 as a transcription activator is involved in the some liver genes transcription. ONECUT1 is highly expressed in liver and lower in testis, skin.

Protocols

Product Specific Protocols
WB protocol for ONECUT1 antibody 25137-1-APDownload protocol
IHC protocol for ONECUT1 antibody 25137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Clin Invest

REVERBa couples the circadian clock to hepatic glucocorticoid action.

Authors - Giorgio Caratti
View Article
humanWB

J Virol

Inhibition of Hepatitis B Virus Gene Expression and Replication by Hepatocyte Nuclear Factor 6.

Authors - Ruidong Hao
View Article
humanWB

Virol Sin

Psoralen inhibits hepatitis B viral replication by down-regulating the host transcriptional machinery of viral promoters

Authors - Xinna Ma
View Article