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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

OBFC2B Polyclonal antibody

OBFC2B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14809-1-AP

Synonyms

hSSB1, OBFC2B, Sensor of ssDNA subunit B1, SOSS B1, SOSS complex subunit B1, SSB1

Validation Data Gallery

Applications:
  • WB analysis using 14809-1-AP

    WB analysis using 14809-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 14809-1-AP

    WB analysis of HepG2 using 14809-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 14809-1-AP

    WB analysis of NIH/3T3 using 14809-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 14809-1-AP

    IP experiment of HeLa using 14809-1-AP

    IP result of anti-OBFC2B (IP:14809-1-AP, 4ug; Detection:14809-1-AP 1:500) with HeLa cells lysate 3200ug.

  • IP experiment of HeLa using 14809-1-AP

    IP experiment of HeLa using 14809-1-AP

    IP result of anti-OBFC2B (IP:14809-1-AP, 4ug; Detection:14809-1-AP 1:500) with HeLa cells lysate 3200ug.

  • IHC staining of human ovary tumor using 14809-1-AP

    IHC staining of human ovary tumor using 14809-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human pancreas using 14809-1-AP

    IHC staining of human pancreas using 14809-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas using 14809-1-AP

    IHC staining of human pancreas using 14809-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human ovary tumor using 14809-1-AP

    IHC staining of human ovary tumor using 14809-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HeLa using 14809-1-AP

    IF Staining of HeLa using 14809-1-AP

    Immunofluorescent analysis of Hela cells, using OBFC2B antibody 14809-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IF Staining of HeLa using 14809-1-AP

    IF Staining of HeLa using 14809-1-AP

    Immunofluorescent analysis of Hela cells, using OBFC2B antibody 14809-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHepG2 cells, NIH/3T3 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14809-1-AP targets OBFC2B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OBFC2B fusion protein Ag6532 相同性解析による交差性が予測される生物種
Full Name oligonucleotide/oligosaccharide-binding fold containing 2B
Calculated molecular weight 22 kDa
Observed molecular weight 30-33 kDa
GenBank accession numberBC001079
Gene symbol OBFC2B
Gene ID (NCBI) 79035
RRIDAB_2251673
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OBFC2B, also named as NABP2 or SSB1, is a 211 amino acid protein, which belongs to the SOSS-B family. OBFC2B localizes to nuclear foci following DNA damage. OBFC2B is a component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, OBFC2B acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The calculated molecular weight of OBFC2B is 22 kDa, but modified OBFC2B is about 32 kDa.

Protocols

Product Specific Protocols
WB protocol for OBFC2B antibody 14809-1-APDownload protocol
IHC protocol for OBFC2B antibody 14809-1-APDownload protocol
IF protocol for OBFC2B antibody 14809-1-APDownload protocol
IP protocol for OBFC2B antibody 14809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

The Integrator complex controls the termination of transcription at diverse classes of gene targets.

Authors - Jeffrey R Skaar
  • KD Validated
View Article
humanWB

Cell Death Dis

Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability.

Authors - Anaïs Aulas
View Article
humanWB,IHC

J Inflamm Res

Comprehensive Analysis of NABP2 as a Prognostic Biomarker and Its Correlation with Immune Infiltration in Hepatocellular Carcinoma

Authors - Bowen Li
View Article