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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Nlrp3 Polyclonal antibody

Nlrp3 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 30109-1-AP

Synonyms

AGTAVPRL, AII/AVP, Cias1, FCAS, FCU, Mmig1, MWS, NALP3, Nlrp3, Pypaf1

Validation Data Gallery

Applications:
  • WB analysis using 30109-1-AP

    WB analysis using 30109-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30109-1-AP (Nlrp3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis using 30109-1-AP

    WB analysis using 30109-1-AP

    Non-treated RAW 264.7 cells and LPS-treated RAW 264.7 cells lysates were subjected to SDS PAGE followed by western blot with 30109-1-AP (Nlrp3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30109-1-AP

    WB analysis using 30109-1-AP

    rat spleen tissue was subjected to SDS PAGE followed by western blot with 30109-1-AP (Nlrp3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 30109-1-AP

    WB analysis using 30109-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30109-1-AP (Nlrp3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of RAW 264.7 using 30109-1-AP

    IP experiment of RAW 264.7 using 30109-1-AP

    IP result of anti-Nlrp3 (IP:30109-1-AP, 4ug; Detection:30109-1-AP 1:4000) with RAW 264.7 cells lysate 1200 ug.

  • IF Staining of HepG2 using 30109-1-AP

    IF Staining of HepG2 using 30109-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Nlrp3 antibody (30109-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJ774A.1 cells, rat spleen tissue, RAW 264.7 cells, THP-1 cells, LPS treated RAW 264.7 cells
Positive IP detected inRAW 264.7 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

30109-1-AP targets Nlrp3 in WB, IP, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Nlrp3 fusion protein Ag32515 相同性解析による交差性が予測される生物種
Full Name NLR family, pyrin domain containing 3
Calculated molecular weight118kd
Observed molecular weight108-120 kDa
GenBank accession numberNM_145827
Gene symbol Nlrp3
Gene ID (NCBI) 216799
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NLRP3, also named as NALP3, CIASI, C1orf7, PYPAF1, Cryopyrin and MIM606416, belongs to NLR family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NLRP3 inflammasome is expressed in immune cells, including monocytes, macrophages, and dendritic cells. When the NLRP3 inflammasome is activated, the PYD domain of NLRP3 mediates recruitment of an adaptor protein called ASC and the effector protein procaspase-1 to form an NLRP3 inflammasome complex that can cleave inactive procaspase-1 to from active caspase-1. And then the active caspase-1 results in secretion of interleukin (IL)-18 and IL-1β. Activation of the NLRP3 inflammasome is also required for HMGB1 secretion. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (PMID:27669650). NLRP3 has some isoforms with the MW of 106-118 kDa and 75-83 kDa. An additional 130-135 kDa band could also be detected (PMID: 17164409; PMID: 34680443; PMID: 35585627). This antibody is specific for mouse Nlrp3.

Protocols

Product Specific Protocols
WB protocol for Nlrp3 antibody 30109-1-APDownload protocol
IF protocol for Nlrp3 antibody 30109-1-APDownload protocol
IP protocol for Nlrp3 antibody 30109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

JACC Basic Transl Sci

PCSK9 Promotes Hypoxia-Induced EC Pyroptosis by Regulating Smac Mitochondrion-Cytoplasm Translocation in Critical Limb Ischemia

Authors - Meixin Zhang
View Article