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- KD/KO Validated
NUDT21 Polyclonal antibody
NUDT21 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, CoIP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 10322-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HL-60 cells, NIH/3T3 cells, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy NUDT21 Ready-To-Use IHC Kit. NUDT21 primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 9 publications below |
| WB | See 24 publications below |
| IHC | See 4 publications below |
| IF | See 4 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
| ChIP | See 2 publications below |
Product Information
10322-1-AP targets NUDT21 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NUDT21 fusion protein Ag0393 相同性解析による交差性が予測される生物種 |
| Full Name | nudix (nucleoside diphosphate linked moiety X)-type motif 21 |
| Calculated molecular weight | 26 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC001403 |
| Gene symbol | NUDT21 |
| Gene ID (NCBI) | 11051 |
| RRID | AB_2251496 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NUDT21 antibody 10322-1-AP | Download protocol |
| IHC protocol for NUDT21 antibody 10322-1-AP | Download protocol |
| IF protocol for NUDT21 antibody 10322-1-AP | Download protocol |
| IP protocol for NUDT21 antibody 10322-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.
| ||
Mol Cell A Complex of U1 snRNP with Cleavage and Polyadenylation Factors Controls Telescripting, Regulating mRNA Transcription in Human Cells.
| ||
Mol Cell Global Promotion of Alternative Internal Exon Usage by mRNA 3' End Formation Factors. | ||
J Exp Med Downregulation of CFIm25 amplifies dermal fibrosis through alternative polyadenylation. | ||
EMBO J The Mediator subunit MED23 couples H2B mono-ubiquitination to transcriptional control and cell fate determination. |
