Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 10484-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10484-1-AP (NUDT2 antibody) at dilution of 1:4000 incubated at 4 degree celsius over night.
WB analysis using 10484-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10484-1-AP (NUDT2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 10484-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10484-1-AP (NUDT2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 10484-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10484-1-AP (NUDT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 10484-1-AP
IP result of anti-NUDT2 (IP:10484-1-AP, 4ug; Detection:10484-1-AP 1:1000) with HEK-293 cells lysate 2600 ug.
IHC staining of human breast cancer using 10484-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10484-1-AP (NUDT2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10484-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10484-1-AP (NUDT2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10484-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10484-1-AP (NUDT2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10484-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10484-1-AP (NUDT2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

NUDT2 Polyclonal antibody

NUDT2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10484-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Ap4A hydrolase, Ap4Aase, APAH1, Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical], Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse colon tissue, mouse liver tissue, mouse spleen, mouse brain tissue, rat brain tissue, rat liver tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human breast cancer tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 1 publications below

Product Information

10484-1-AP targets NUDT2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0743 Product name: Recombinant human NUDT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-147 aa of BC004926 Sequence: MALRACGLIIFRRCLIPKVDNNAIEFLLLQASDGIHHWTPPKGHVEPGEDDLETALRETQEEAGIEAGQLTIIEGFKRELNYVARNKPKTVIYWLAEVKDYDVEIRLSHEHQAYRWLGLEEACQLAQFKEMKAALQEGHQFLCSIEA 相同性解析による交差性が予測される生物種
Full Name	nudix (nucleoside diphosphate linked moiety X)-type motif 2
Calculated molecular weight	17 kDa
Observed molecular weight	17 kDa
GenBank accession number	BC004926
Gene Symbol	NUDT2
Gene ID (NCBI)	318
RRID	AB_2153978
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P50583
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for NUDT2 antibody 10484-1-AP	Download protocol
IP protocol for NUDT2 antibody 10484-1-AP	Download protocol
WB protocol for NUDT2 antibody 10484-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
Human	WB	Oncol Rep Knockdown of GATAD2A suppresses cell proliferation in thyroid cancer in vitro. Authors - Zongping Wang View Article
human	WB	Int J Mol Sci Role of Nudt2 in Anchorage-Independent Growth and Cell Migration of Human Melanoma Authors - Sana' Hidmi KD Validated View Article
human	IHC	Front Oncol The functional role of Nudt2 in human triple negative breast cancer Authors - Rasha Abu-Rahmah View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10484-1-AP

WB analysis using 10484-1-AP

WB analysis using 10484-1-AP

WB analysis using 10484-1-AP

IP experiment of HEK-293 using 10484-1-AP

IHC staining of human breast cancer using 10484-1-AP

IHC staining of human breast cancer using 10484-1-AP

IHC staining of human liver cancer using 10484-1-AP

IHC staining of human liver cancer using 10484-1-AP

NUDT2 Polyclonal antibody

NUDT2 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Knockdown of GATAD2A suppresses cell proliferation in thyroid cancer in vitro.

Role of Nudt2 in Anchorage-Independent Growth and Cell Migration of Human Melanoma

The functional role of Nudt2 in human triple negative breast cancer