Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 11 publications below |
| IHC | See 8 publications below |
| IF | See 2 publications below |
Product Information
18084-1-AP targets Neurotrophin 3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12798 Product name: Recombinant human NTF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-257 aa of BC069773 Sequence: MSILFYVIFLAYLRGIQGNNMDQRSLPEDSLNSLIIKLIQADILKNKLSKQMVDVKENYQSTLPKAEAPREPERGGPAKSAFQPVIAMDTELLRQQRRYNSPRVLLSDSTPLEPPPLYLMEDYVGSPVVANRTSRRKRYAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT 相同性解析による交差性が予測される生物種 |
| Full Name | neurotrophin 3 |
| Calculated molecular weight | 257 aa, 29 kDa |
| GenBank accession number | BC069773 |
| Gene Symbol | Neurotrophin 3 |
| Gene ID (NCBI) | 4908 |
| RRID | AB_2298715 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20783 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Neurotrophin-3 (NT-3) is a member of the neurotrophin family, which are growth factor proteins that play essential roles in the development and maintenance of the nervous system. NT-3 is synthesized by fibroblasts and has been extensively studied for its role in the development of neurons and neural crest-derived cells, such as melanocytes. It can bind to each of the Trk receptors (Trk-A, Trk-B, and Trk-C), but it primarily functions by binding to Trk-C, modulating intracellular signal transduction through the MAPK and PI3K-Akt pathways, which regulate melanocyte differentiation and survival.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Neurotrophin 3 antibody 18084-1-AP | Download protocol |
| IHC protocol for Neurotrophin 3 antibody 18084-1-AP | Download protocol |
| WB protocol for Neurotrophin 3 antibody 18084-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioact Mater Construction of functional neural network tissue combining CBD-NT3-modified linear-ordered collagen scaffold and TrkC-modified iPSC-derived neural stem cells for spinal cord injury repair | ||
Phytomedicine Naringin reduces mitochondrial fragmentation and excessive mitophagy in cerebral ischemia-reperfusion injury via the S-nitrosylated Dynamin-related protein 1 (SNO-DRP1) / Leucine-rich repeat kinase 2 (LRRK2) / Mitochondrial Calcium Uniporter (MCU) pathway. | ||
J Orthop Translat Schwann-endothelial crosstalk in neurovascularized cell sheets induced host CGRP release to drive mandibular bone regeneration. | ||
ACS Appl Mater Interfaces Surface Coating of Pulmonary siRNA Delivery Vectors Enabling Mucus Penetration, Cell Targeting, and Intracellular Radical Scavenging for Enhanced Acute Lung Injury Therapy. | ||
Stem Cell Res Ther NT3P75-2 gene-modified bone mesenchymal stem cells improve neurological function recovery in mouse TBI model. | ||
Transl Psychiatry The causal involvement of the BDNF-TrkB pathway in dentate gyrus in early-life stress-induced cognitive deficits in male mice |