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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated NTF2 Monoclonal antibody

NTF2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4H7F9

Cat no : CL488-66063

Synonyms

NTF 2, NTF2, nuclear transport factor 2, NUTF2, Placental protein 15, PP15

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-66063

    IF Staining of HeLa using CL488-66063

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488-conjugated NTF2 antibody (CL488-66063, Clone: 4H7F9 ) at dilution of 1:200, CL594-Phalloidin (red).

  • IF Staining of HeLa using CL488-66063

    IF Staining of HeLa using CL488-66063

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66063 (NTF2 antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66063-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-66063 is NTF2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NTF2 fusion protein Ag7879 相同性解析による交差性が予測される生物種
Full Name nuclear transport factor 2
Calculated molecular weight 14 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC002348
Gene symbol NUTF2
Gene ID (NCBI) 10204
RRIDAB_2883246
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NUTF2 (nuclear transport factor 2), also named as NTF2, is a cytosolic protein responsible for nuclear import of Ran, a small Ras-like GTPase involved in a number of critical cellular processes, including cell cycle regulation, chromatin organization during mitosis, reformation of the nuclear envelope following mitosis, and controlling the directionality of nucleocytoplasmic transport. Nucleocytoplasmic translocation of NTF2 is regulated in mammalian cells, and may involve a tyrosine and/or threonine kinase-dependent signal transduction mechanism(s) (PMID: 22880006). The MW of this protein is 14 kDa, and this antibody specially recognises the 14 kDa protein.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NTF2 antibody CL488-66063Download protocol
Standard Protocols
Click here to view our Standard Protocols