Validation Data Gallery

Filter: AllWBIHCIF-P

WB analysis using 12231-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12231-1-AP (CD73 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12231-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12231-1-AP (CD73 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 12231-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 12231-1-AP (CD73 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human spleen using 12231-1-AP
human spleen tissue were subjected to SDS PAGE followed by western blot with 12231-1-AP (CD73 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12231-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12231-1-AP (CD73 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 12231-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 12231-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12231-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12231-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12231-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 12231-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 12231-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 12231-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 12231-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 12231-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 12231-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse small intestine using 12231-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CD73 antibody (12231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse small intestine using 12231-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CD73 antibody (12231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human appendicitis using 12231-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using CD73 antibody (12231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

CD73 Polyclonal antibody

CD73 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 12231-1-AP

Publications(59)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Synonyms

NT5E, 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), 5'-deoxynucleotidase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (59)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A375 cells, human spleen tissue, K-562 cells, mouse brain tissue, HT-29 cells, HuH-7 cells, Jurkat cells
Positive IHC detected in	human lung cancer tissue, human stomach cancer tissue, human stomach tissue, mouse kidney tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse small intestine tissue, human appendicitis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:3000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 29 publications below
IHC	See 17 publications below
IF	See 20 publications below
ELISA	See 1 publications below

Product Information

12231-1-AP targets CD73 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2869 Product name: Recombinant human NT5E,CD73 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 32-264 aa of BC015940 Sequence: LHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNCFKRIAWARMSR 相同性解析による交差性が予測される生物種
Full Name	5'-nucleotidase, ecto (CD73)
Calculated molecular weight	29 kDa, 63 kDa
Observed molecular weight	60-70 kDa
GenBank accession number	BC015940
Gene Symbol	CD73
Gene ID (NCBI)	4907
RRID	AB_2154081
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P21589
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NT5E(5'-nucleotidase) is also named as NT5, NTE,CD73,ecto-5'-nucleotidase, and belongs to the 5'-nucleotidase family. It catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP.The enzyme occurs mainly as a dimer and the wide distribution of NT5E in the rat hippocampus, suggesting their involvement in the control of the purinergic signaling(PMID:17619139). This protein has two isoforms with the molecular weight of 63 kDa and 58 kDa and four glycosylation sites with a signalpeptide. It can exsit as a dimer(PMID:17619139). It can be detected the band of 55 kDa, 64 kDa, 70 kDa, 110 kDa by western blot(PMID:17619139; 12370277; 21533188). Defects in NT5E are the cause of calcification of joints and arteries (CALJA).

Protocols

Product Specific Protocols
IF protocol for CD73 antibody 12231-1-AP	Download protocol
IHC protocol for CD73 antibody 12231-1-AP	Download protocol
WB protocol for CD73 antibody 12231-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Adv Healthc Mater Dual-Barb Microneedle with JAK/STAT Inhibitor-Loaded Nanovesicles Encapsulation for Tendinopathy Authors - Minhao Chen View Article
mouse,human	WB,IHC,IF	Int J Biol Sci CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence Authors - Zhenni Liu View Article
human	WB,IHC	Br J Cancer Norepinephrine inhibits CD8+ T-cell infiltration and function, inducing anti-PD-1 mAb resistance in lung adenocarcinoma Authors - Qishun Geng View Article
mouse	IF	Theranostics GATA4-driven miR-206-3p signatures control orofacial bone development by regulating osteogenic and osteoclastic activity. Authors - Shuyu Guo View Article
human	IF	Cell Mol Biol Lett Reciprocal negative feedback between Prrx1 and miR-140-3p regulates rapid chondrogenesis in the regenerating antler Authors - Pengfei Hu View Article
	IHC	Theranostics A sericin/ graphene oxide composite scaffold as a biomimetic extracellular matrix for structural and functional repair of calvarial bone. Authors - Chao Qi View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12231-1-AP

WB analysis of mouse brain using 12231-1-AP

WB analysis of A375 using 12231-1-AP

WB analysis of human spleen using 12231-1-AP

WB analysis of K-562 using 12231-1-AP

IHC staining of human colon cancer using 12231-1-AP

IHC staining of human stomach cancer using 12231-1-AP

IHC staining of human lung cancer using 12231-1-AP

IHC staining of human lung cancer using 12231-1-AP

IHC staining of human colon cancer using 12231-1-AP

IHC staining of human stomach cancer using 12231-1-AP

IHC staining of mouse kidney using 12231-1-AP

IHC staining of mouse kidney using 12231-1-AP

IHC staining of human stomach using 12231-1-AP

IHC staining of human stomach using 12231-1-AP

IHC staining of human stomach using 12231-1-AP

IF Staining of mouse small intestine using 12231-1-AP

IF Staining of mouse small intestine using 12231-1-AP

IF Staining of human appendicitis using 12231-1-AP

CD73 Polyclonal antibody

CD73 Polyclonal Antibody for WB, IHC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Dual-Barb Microneedle with JAK/STAT Inhibitor-Loaded Nanovesicles Encapsulation for Tendinopathy

CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence

Norepinephrine inhibits CD8+ T-cell infiltration and function, inducing anti-PD-1 mAb resistance in lung adenocarcinoma

GATA4-driven miR-206-3p signatures control orofacial bone development by regulating osteogenic and osteoclastic activity.

Reciprocal negative feedback between Prrx1 and miR-140-3p regulates rapid chondrogenesis in the regenerating antler

A sericin/ graphene oxide composite scaffold as a biomimetic extracellular matrix for structural and functional repair of calvarial bone.